The first two authors contributed equally to this work.
Weissellicin L, a novel bacteriocin from sian-sianzih-isolated Weissella hellenica 4-7
Article first published online: 15 MAY 2013
Journal of Applied Microbiology © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 1, pages 70–76, July 2013
How to Cite
Leong, K.-H., Chen, Y.-S., Lin, Y.-H., Pan, S.-F., Yu, B., Wu, H.-C. and Yanagida, F. (2013), Weissellicin L, a novel bacteriocin from sian-sianzih-isolated Weissella hellenica 4-7. Journal of Applied Microbiology, 115: 70–76. doi: 10.1111/jam.12218
- Issue published online: 12 JUN 2013
- Article first published online: 15 MAY 2013
- Accepted manuscript online: 18 APR 2013 03:34AM EST
- Manuscript Accepted: 9 APR 2013
- Manuscript Revised: 8 APR 2013
- Manuscript Received: 30 JAN 2013
- fermented clams;
- lactic acid bacteria;
- Weissella hellenica ;
To identify and characterize novel bacteriocins from Weissella hellenica 4-7.
Methods and Results
Weissella hellenica 4-7, isolated from the traditional Taiwanese fermented food sian-sianzih (fermented clams), was previously found to produce a bacteriocin active against Listeria monocytogenes and some other Gram-positive bacteria. Bacteriocin activity decreased slightly after autoclaving (121°C for 15 min), but was inactivated by protease K and trypsin. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 3205·6 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-KGFLSWASKATSWLVGP, by Edman degradation. The obtained partial sequence showed high homology with leucocin B-TA33a; however, at least two different residues were observed. No identical peptide or protein was found, and this peptide was therefore considered to be a novel bacteriocin produced by W. hellenica 4-7 and termed weissellicin L.
The findings obtained in the current study suggest a novel bacteriocin produced by W. hellenica 4-7.
Significance and Impact of the Study
Bacteriocins from Weissella remain rare, and this study is the second report of a bacteriocin produced by W. hellenica.