Subinhibitory concentrations of pinocembrin exert anti-Staphylococcus aureus activity by reducing α-toxin expression

Authors

  • L.W. Soromou,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
    2. Département de Médecine Vétérinaire, Institut Supérieur des Sciences et de Médecine Vétérinaire (ISSMV) de Dalaba, Dalaba, République de Guinée
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    • These authors contributed equally to this work.
  • Y. Zhang,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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    • These authors contributed equally to this work.
  • Y. Cui,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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  • M. Wei,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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  • N. Chen,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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  • X. Yang,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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  • M. Huo,

    1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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  • A. Baldé,

    1. Département de Médecine Vétérinaire, Institut Supérieur des Sciences et de Médecine Vétérinaire (ISSMV) de Dalaba, Dalaba, République de Guinée
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  • S. Guan,

    1. Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, China
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  • X. Deng,

    Corresponding author
    • Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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  • D. Wang

    Corresponding author
    • Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China
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Correspondence

Xuming Deng, Dacheng Wang, Department of veterinary pharmacology, Jilin University, Changchun 130062, China. E-mails: xumingdeng@jluhp.edu.cn; wdc9928@yahoo.com.cn

Abstract

Aims

Natural products have been used as potentially important sources of novel antibacterials in combating pathogenic Staphylococcus aureus isolates, a major problem around the world. In this study, we aimed to investigate the antibacterial effects of pinocembrin (PNCB) against Staph. aureus pneumonia in a murine model and its influence on the production of Staph. aureus α-haemolysin (Hla).

Methods and Results

The in vitro activities of PNCB on α-haemolysin production were determined using haemolysis, Western blot and real-time RT-PCR assays. The viability and cytotoxicity assays were performed to evaluate the influence of PNCB on α-toxin-mediated injury of human alveolar epithelial cells. Moreover, through histopathologic analysis, we further determined the in vivo effects of PNCB on Staph. aureus pneumonia in a mouse model. In vitro, PNCB at low concentrations exhibited inhibitory activity against α-haemolysin production and attenuated α-haemolysin-mediated cell injury. Furthermore, the in vivo findings demonstrated that PNCB protected mice from Staph. aureus pneumonia.

Conclusions

We have provided new evidence of the effects of PNCB, which suggest that PNCB attenuated α-haemolysin-mediated cell injury and protected mice from Staph. aureus pneumonia.

Significance and Impact of the Study

The findings indicate that PNCB may be used as a basis for anti-Staphylococcus agent.

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