Structural and functional study in the rhizosphere of Oryza sativa L. plants growing under biotic and abiotic stress
Article first published online: 16 MAY 2013
Journal of Applied Microbiology © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 1, pages 218–235, July 2013
How to Cite
Lucas, J.A., García-Villaraco, A., Ramos, B., García-Cristobal, J., Algar, E. and Gutierrez-Mañero, J. (2013), Structural and functional study in the rhizosphere of Oryza sativa L. plants growing under biotic and abiotic stress. Journal of Applied Microbiology, 115: 218–235. doi: 10.1111/jam.12225
- Issue published online: 12 JUN 2013
- Article first published online: 16 MAY 2013
- Accepted manuscript online: 18 APR 2013 03:34AM EST
- Manuscript Accepted: 8 APR 2013
- Manuscript Revised: 21 MAR 2013
- Manuscript Received: 4 FEB 2013
- Ministerio de Ciencia e Innovación. Grant Numbers: AGL2006-13758-C05-02, AGL2009-13487-C04-04
- Comunidad de Madrid. Grant Numbers: CAM S-0505/AMB/000321, S2009/AMB-1511
- 16S rRNA libraries;
- microbial functional diversity;
- plant growth–promoting rhizobacteria;
- rhizobacteria biodiversity;
- stress conditions
A structural and functional study has been carried out in the rice production area of the Guadalquivir marshes in southern Spain aiming to increase knowledge of rice rhizosphere structure and function for further application on integrated management practices.
Methods and Results
Rhizosphere bacterial structure (analysis of 16S rRNA partial sequences from total soil DNA), metabolic diversity (analysed by Biolog FF for fungal community and GN for microbial community) and a screening for putative plant growth–promoting rhizobacteria (PGPR) to identify potential isolates for development of local biofertilizers, and biodiversity of culturable micro-organisms (analysis of 16S rRNA partial sequences) from four areas differing in salinity and Magnaporthe oryzae incidence in two moments of the crop cycle were studied. Results indicate that the dominant taxon in libraries from the four areas was Proteobacteria. Metabolic diversity was higher in areas affected only by salinity or incidence of Magnaporthe than in the control or area affected by both stresses. It seems that rice plants selected, in their rhizosphere, micro-organisms able to affect plant hormonal balance under all conditions, and this activity relied in different bacterial genera depending on the environmental stress.
Bacterial genera for each stress, as well as generalist strains, were found present in all the studied areas. Potential molecular markers and taxonomic markers (Sphingobacteria for salt and Thermococci for Magnaporthe) of the different stress situations have been highlighted, and Class Verrucomicrobiae could be a marker for nonstressed areas. In addition, putative PGPR strains isolated in this study could be used as biofertilizers.
Significance and Impact of the Study
Rice paddies are great ecologically important ecosystems. The results are very relevant as they may be included in the process of rice production, improving crop conditions with less environmental impact.