Identification and characterization of novel multiple bacteriocins produced by Lactobacillus sakei D98
Article first published online: 16 MAY 2013
Journal of Applied Microbiology © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 1, pages 61–69, July 2013
How to Cite
Sawa, N., Koga, S., Okamura, K., Ishibashi, N., Zendo, T. and Sonomoto, K. (2013), Identification and characterization of novel multiple bacteriocins produced by Lactobacillus sakei D98. Journal of Applied Microbiology, 115: 61–69. doi: 10.1111/jam.12226
- Issue published online: 12 JUN 2013
- Article first published online: 16 MAY 2013
- Accepted manuscript online: 18 APR 2013 03:34AM EST
- Manuscript Accepted: 8 APR 2013
- Manuscript Revised: 18 MAR 2013
- Manuscript Received: 18 DEC 2012
- Japan Society for the Promotion of Science
- lactic acid bacteria;
- Lactobacillus sakei ;
- multiple bacteriocins;
To characterize novel multiple bacteriocins produced by Lactobacillus sakei D98.
Methods and Results
Lactobacillus sakei D98 isolated from Shubo (rice malt) produced at least three bacteriocins. Using three purification steps, three novel antimicrobial peptides termed sakacin D98a, sakacin D98b and sakacin D98c were purified from the culture supernatant. Amino acid and DNA sequencing analysis revealed that the sakacins D98a, D98b and D98c are novel class IIa-like or class IId bacteriocins. In particular, sakacin D98b has a variant pediocin-box sequence, YANGVXC (with Ala instead of Gly), and a different location for the disulfide bridge (Cys11 and Cys18) from that found in other class IIa bacteriocins.
Three novel bacteriocins were identified from Lactobacillus sakei D98. Their antimicrobial spectra and intensities indicate that these sakacins would have different modes of action. In addition, sakacin D98b showed low inhibitory activity against Listeria, probably due to the differences in amino acids and position of the disulfide bridge compared with the other class IIa bacteriocins.
Significance and Impact of Study
Sakacins D98a and D98c are novel bacteriocins belonging to class IId bacteriocins. On the other hand, sakacin D98b, a class IIa-like bacteriocin, has a unique internal structure and activity spectrum.