Evaluation of characteristics of Pediococcus spp. to be used as a vaginal probiotic
Article first published online: 16 MAY 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 2, pages 527–538, August 2013
How to Cite
Borges, S., Barbosa, J., Silva, J. and Teixeira, P. (2013), Evaluation of characteristics of Pediococcus spp. to be used as a vaginal probiotic. Journal of Applied Microbiology, 115: 527–538. doi: 10.1111/jam.12232
- Issue published online: 17 JUL 2013
- Article first published online: 16 MAY 2013
- Accepted manuscript online: 23 APR 2013 07:41AM EST
- Manuscript Accepted: 17 APR 2013
- Manuscript Revised: 23 JAN 2013
- Manuscript Received: 29 NOV 2012
- FCT – Fundação para a Ciência e a Tecnologia. Grant Numbers: PEst-OE/EQB/LA0016/2011, SFRH/BD/45496/2008
- antibiotic susceptibility;
- antimicrobial activity;
- Listeria monocytogenes ;
- vaginal fluid;
- vaginal probiotic;
- virulence factors
The aim of our research was to select, identify and characterize an isolate of lactic acid bacteria to be considered as a vaginal probiotic.
Methods and Results
Thirty-five isolates of Pediococcus spp. showed bacteriocinogenic activity against Listeria monocytogenes and the ability to survive in simulated vaginal fluid (SVF) at pH 4·2. One isolate of Pediococcus spp. was selected and characterized to evaluate its safety before the use as vaginal probiotic. Pediococcus pentosaceus SB83 did not show the presence of virulence factors such as the production of gelatinase, lipase and DNase, haemolytic activity, nor the presence of virulence genes (genes esp, agg, gelE, efaAfm, efaAfs, cylA, cylB and cylM). Pediococcus pentosaceus SB83 was considered sensitive to chloramphenicol, gentamicin, streptomycin, kanamycin, erythromycin and ampicillin. This strain was considered resistant to tetracycline and vancomycin. Pediococcus pentosaceus SB83 was a biofilm producer at different pH values (4·2, 5·5 and 6·5) in SVF and in de Man, Rogosa and Sharpe medium.
The in vitro results provide a basis for the use of P. pentosaceus SB83 as a vaginal probiotic, to prevent colonization by L. monocytogenes in pregnant women.
Significance and Impact of Study
The application of vaginal probiotics could have the potential for preventing vaginal infections and consequently reduce abortion and neonatal infections.