The effect of growth media and physical treatments on the adhesion properties of canine probiotics
Article first published online: 21 MAY 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 2, pages 539–545, August 2013
How to Cite
Grześkowiak, Ł., Endo, A., Collado, M.C., Pelliniemi, L.J., Beasley, S. and Salminen, S. (2013), The effect of growth media and physical treatments on the adhesion properties of canine probiotics. Journal of Applied Microbiology, 115: 539–545. doi: 10.1111/jam.12235
- Issue published online: 17 JUL 2013
- Article first published online: 21 MAY 2013
- Accepted manuscript online: 26 APR 2013 01:12AM EST
- Manuscript Accepted: 19 APR 2013
- Manuscript Revised: 18 APR 2013
- Manuscript Received: 18 JAN 2013
- growth media;
- Lactobacillus ;
The manufacturing processes have been reported to influence the properties of probiotics with potential impact on health properties. The aim was to investigate the effect of different growth media and inactivation methods on the properties of canine-originated probiotic bacteria alone and in combination mixture.
Methods and Results
Three established dog probiotics, Lactobacillus fermentum VET9A, Lactobacillus plantarum VET14A and Lactobacillus rhamnosus VET16A, and their combination mixture were evaluated for their adhesion to dog mucus. The effect of different growth media, one reflecting laboratory and the other manufacturing conditions, and inactivation methods (95°C, 80°C and UV irradiation) on the mucus adhesion of the probiotic strains was characterized. Evaluation of dog probiotics was supported by cell visualization using transmission electron microscopy (TEM). Higher adhesion percentage was reported for probiotic strains growing in laboratory rather than in manufacturing conditions (P < 0·05). Inactivation by heat (95°C, 80°C) decreased the adhesion properties when strains were cultivated in soy-based growth media compared with those grown in MRS broth (P < 0·05). TEM observations uncovered differences in cell-surface components in nonviable forms of probiotic strains as compared with their viable forms.
Manufacturing process conditions such as growth media and pretreatment methods may significantly affect the adhesive ability of the tested strains.
Significance and Impact of the Study
Growth conditions, growth media, pretreatment methods and different probiotic combinations should be carefully considered for quality control of existing probiotics and for identification of new probiotics for dogs. These may also have an impact on health benefits for the host.