Enhanced inhibition of Aspergillus niger on sedge (Lepironia articulata) treated with heat-cured lime oil
Article first published online: 21 MAY 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 2, pages 376–381, August 2013
How to Cite
Matan, N., Matan, N. and Ketsa, S. (2013), Enhanced inhibition of Aspergillus niger on sedge (Lepironia articulata) treated with heat-cured lime oil. Journal of Applied Microbiology, 115: 376–381. doi: 10.1111/jam.12236
- Issue published online: 17 JUL 2013
- Article first published online: 21 MAY 2013
- Accepted manuscript online: 26 APR 2013 01:12AM EST
- Manuscript Accepted: 19 APR 2013
- Manuscript Revised: 12 APR 2013
- Manuscript Received: 15 JAN 2013
- Office of the Higher Education Commission
- Ministry of Education
- Thailand Research Fund
- Walailak University
- Thailand Toray Science Foundation
- lime oil;
- heat curing;
- Aspergillus niger ;
- sedge (Lepironia articulata)
This study aimed to examine heat curing effect (30–100°C) on antifungal activities of lime oil and its components (limonene, p-cymene, β-pinene and α-pinene) at concentrations ranging from 100 to 300 μl ml−1 against Aspergillus niger in microbiological medium and to optimize heat curing of lime oil for efficient mould control on sedge (Lepironia articulata).
Methods and Results
Broth dilution method was employed to determine lime oil minimum inhibitory concentration, which was at 90 μl ml−1 with heat curing at 70°C. Limonene, a main component of lime oil, was an agent responsible for temperature dependencies of lime oil activities observed. Response surface methodology was used to construct the mathematical model describing a time period of zero mould growth on sedge as functions of heat curing temperature and lime oil concentration. Heat curing of 90 μl ml−1 lime oil at 70°C extended a period of zero mould growth on sedge to 18 weeks under moist conditions.
Heat curing at 70°C best enhanced antifungal activity of lime oil against A. niger both in medium and on sedge.
Significance and Impact of the Study
Heat curing of lime oil has potential to be used to enhance the antifungal safety of sedge products.