The expression of adhesin EF-Tu in response to mucin and its role in Lactobacillus adhesion and competitive inhibition of enteropathogens to mucin
Article first published online: 3 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 2, pages 546–554, August 2013
How to Cite
Dhanani, A.S. and Bagchi, T. (2013), The expression of adhesin EF-Tu in response to mucin and its role in Lactobacillus adhesion and competitive inhibition of enteropathogens to mucin. Journal of Applied Microbiology, 115: 546–554. doi: 10.1111/jam.12249
- Issue published online: 17 JUL 2013
- Article first published online: 3 JUN 2013
- Accepted manuscript online: 11 MAY 2013 10:27AM EST
- Manuscript Revised: 8 MAY 2013
- Manuscript Accepted: 8 MAY 2013
- Manuscript Received: 18 MAR 2013
- Department of Biotechnology. Grant Number: BT/PR-7496/PID/20/292/2006
- University Grant Commission
- competitive adhesion;
- Lactobacillus ;
- mucin adhesion;
To analyse the expression of EF-Tu in Lactobacillus strains with response to mucin exposure and its role in interfering with adhesion of enteropathogens to mucin.
Methods and Results
The Lactobacillus strains were analysed for their ability to adhere to immobilized mucin in microtiter plates. Lactobacillus delbrueckii M and Lactobacillus plantarum CS24·2 showed statistically significant adhesion to mucin, which was similar to Lactobacillus rhamnosus GG, the best binding probiotic strain. Lactobacillus rhamnosus GG, Lact. delbrueckii M, Lact. plantarum CS23 and Lact. plantarum CS24·2 were able to effectively antagonize the adhesion of Escherichia coli and Salmonella enterica serovar Typhi to mucin. In the presence of Lactobacillus adhesin – EF-Tu, the adhesion of Lact. delbrueckii M and the strains of Lact. plantarum to mucin was significantly inhibited. Similarly, EF-Tu also reduced the adhesion of enteropathogens to mucin. Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of EF-Tu gene in the strains of Lact. plantarum and Lact. delbrueckii M when exposed to mucin for 3 h.
The study shows the significant role of EF-Tu in lactobacilli adhesion and enteropathogens inhibition.
Significance and Impact of the Study
The study suggests EF-Tu as an important factor linked to the Lactobacillus adhesion as well as enteropathogen inhibition. Lactobacillus plantarum CS23 and Lact. plantarum CS24·2 can be used as potential probiotic strains.