These authors have the same contribution to this work.
Bioconversion of elephant grass (Pennisetum purpureum) acid hydrolysate to bacterial cellulose by Gluconacetobacter xylinus
Article first published online: 24 JUL 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 4, pages 995–1002, October 2013
How to Cite
Yang, X.-Y., Huang, C., Guo, H.-J., Xiong, L., Li, Y.-Y., Zhang, H.-R. and Chen, X.-D. (2013), Bioconversion of elephant grass (Pennisetum purpureum) acid hydrolysate to bacterial cellulose by Gluconacetobacter xylinus. Journal of Applied Microbiology, 115: 995–1002. doi: 10.1111/jam.12255
- Issue published online: 16 SEP 2013
- Article first published online: 24 JUL 2013
- Manuscript Accepted: 15 MAY 2013
- Manuscript Revised: 28 APR 2013
- Manuscript Received: 7 MAR 2013
- Natural Science Foundation of Guangdong Province. Grant Number: S2012040007546
- Support Plan Project of National Science and Technology. Grant Number: 2012BAD32B07
- National Natural Science Foundation of China. Grant Number: U1261116
- Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences. Grant Number: y107rf1001
- acid hydrolysate;
- bacterial cellulose;
- elephant grass;
- Gluconacetobacter xylinus
To evaluate the possibility of elephant grass acid hydrolysate converting into bacterial cellulose (BC) produced by Gluconacetobacter xylinus CH001 and to characterize the morphology and structure of the cellulose produced.
Methods and Results
Acid-hydrolysed and detoxified elephant grass acid hydrolysate was inoculated with G. xylinus CH001. After 14 days of static fermentation, about 6·4 g l−1 of BC could be generated. Meanwhile, 60·4% (w/w) of BC yield on sugar consumption was obtained. Scanning electron micrographs illustrated that the network of cellulose fibres became denser, and the diameter changed with the growth. FT-IR spectra showed almost same results for all the BC samples collected on different culture time. X-ray diffractograms demonstrated that the crystalline form of BC was cellulose I, the crystallinity increased to 53·58%, and the crystallinity index reached up to 99%.
Elephant grass acid hydrolysate could be utilized efficiently for BC production by G. xylinus CH001. Structure analysis on the cellulose produced showed its potential of being excellent material for further application.
Significance and Impact of the Study
Our studies for the first time examined the bioconversion of low-cost elephant grass into high-value BC and the changes in its morphology and structure following the culture time.