Isolation and identification of a gallotannin 1,2,6-tri-O-galloyl-β-d-glucopyranose from hydroalcoholic extract of Terminalia chebula fruits effective against multidrug-resistant uropathogens
Article first published online: 10 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 2, pages 390–397, August 2013
How to Cite
Bag, A., Bhattacharyya, S.K. and Chattopadhyay, R.R. (2013), Isolation and identification of a gallotannin 1,2,6-tri-O-galloyl-β-d-glucopyranose from hydroalcoholic extract of Terminalia chebula fruits effective against multidrug-resistant uropathogens. Journal of Applied Microbiology, 115: 390–397. doi: 10.1111/jam.12256
- Issue published online: 17 JUL 2013
- Article first published online: 10 JUN 2013
- Accepted manuscript online: 18 MAY 2013 08:55AM EST
- Manuscript Revised: 15 MAY 2013
- Manuscript Accepted: 15 MAY 2013
- Manuscript Received: 1 MAR 2013
- Indian Statistical Institute, Kolkata, India. Grant Number: 5613 2008-2011
- antibacterial activity;
- drug resistance;
- mechanism of action;
- Terminalia chebula ;
- urinary tract infections
In this study, an attempt has been made to isolate and identify the bioactive compounds from hydroalcoholic extract of Terminalia chebula fruits effective against multidrug-resistant uropathogens and also to elucidate the influence of metal ions on the growth inhibitory activity of isolated compounds against the studied bacteria, if any.
Methods and Results
Bioassay-guided fractionation and extensive spectrometric analyses (FT-IR, 1H NMR, 13C NMR and ESI-MS) were used to isolate and characterize the bioactive compound. Growth inhibitory activities of isolated compound were studied by agar well diffusion and microbroth dilution assay methods. Checkerboard titration method was used for combination study between antibiotics and isolated compound. Influence of metal ions on growth inhibitory activity of this bioactive compound against the test isolates were also studied by INT [P-iodonitrotetrazolium violet; 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride] colorimetric assay. The isolated bioactive compound 1, 2, 6-tri-O-galloyl-β-d-glucopyranose was found to be responsible for antibacterial activity against multidrug-resistant uropathogens and showed synergy with trimethoprim and gentamicin. This antibacterial activity of bioactive compound was counteracted by the supplementation of iron in the medium.
Terminalia chebula fruit extract contains bioactive compound effective against multidrug-resistant uropathogens, and this antibacterial activity may be due to its iron-complexing property.
Significance and Impact of the Study
To the best of our knowledge, the antibacterial activity exhibited by isolated gallotannin against multidrug-resistant uropathogens is first time reported by us. Besides, these promising findings may lead to the development of antimicrobial agents from T. chebula fruits for the treatment of urinary tract infections caused by these pathogens.