Vertical profiles of community and activity of methanotrophs in landfill cover soils of different age
Article first published online: 19 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 3, pages 756–765, September 2013
How to Cite
Kong, J.-Y., Su, Y., Zhang, Q.-Q., Bai, Y., Xia, F.-F., Fang, C.-R. and He, R. (2013), Vertical profiles of community and activity of methanotrophs in landfill cover soils of different age. Journal of Applied Microbiology, 115: 756–765. doi: 10.1111/jam.12263
- Issue published online: 14 AUG 2013
- Article first published online: 19 JUN 2013
- Accepted manuscript online: 31 MAY 2013 09:50PM EST
- Manuscript Accepted: 24 MAY 2013
- Manuscript Revised: 29 APR 2013
- Manuscript Received: 12 MAR 2013
- Zhejiang Province Natural Science Foundation for Distinguished Young Scholars. Grant Number: R13E080004
- Fundamental Research Funds for the Central Universities. Grant Number: 2012QNA6006
- CH4 oxidation activity;
- landfill age;
- landfill cover soil;
- microbial community
Aerobic CH4 oxidation is an important process controlling CH4 release from landfills to the atmosphere. The aim of this study was to investigate the link between CH4 oxidation activity and methanotrophs abundance and diversity in landfill cover soils of different age.
Methods and Results
Among the three investigated sites, the highest CH4 emission occurred at the active landfill area with the range of 1371–2242 mg m−2 day. The CH4 oxidation activities of landfill cover soils were 1·07–1·21 μmol g−1 h−1 in the landfill area of 7–16 years, which was 7–17 times higher than those in the active landfill area. The relative abundance of methanotrophs assessed by quantification of pmoA gene was about 1·7 × 106–2·4 × 107 copies g−1 in the landfill cover soils. The CH4 oxidation activity was positively correlated with pmoA copy number in the landfill cover soil of each site, respectively. Type II methanotrophs (Methylocystis) and type I methanotrophs including Methylosoma, Methylocaldum and Methylococcus were all present in the landfill cover soils. Compared to type I methanotroph, type II methanotroph, Methylocystis, was more abundant in the acidic landfill cover soils.
Oxidation activity and community structure of methanotrophs varied with depth and age of landfill cover soils.
Significance and Impact of the Study
These findings provide new fundamental information regarding the activity and diversity of methanotrophs in landfill cover soils of different age that may aid predicting and modelling CH4 flux from landfills.