Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family
Article first published online: 28 JUN 2013
© 2013 The Authors. Journal of Applied Microbiology published by Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Journal of Applied Microbiology
Volume 115, Issue 3, pages 835–847, September 2013
How to Cite
Purohit, A.A., Johansen, J. A., Hansen, H., Leiros, H.-K.S., Kashulin, A., Karlsen, C., Smalås, A., Haugen, P. and Willassen, N.P. (2013), Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family. Journal of Applied Microbiology, 115: 835–847. doi: 10.1111/jam.12264
- Issue published online: 14 AUG 2013
- Article first published online: 28 JUN 2013
- Accepted manuscript online: 31 MAY 2013 09:51PM EST
- Manuscript Accepted: 25 MAY 2013
- Manuscript Revised: 10 MAY 2013
- Manuscript Received: 26 FEB 2013
- acyl-homoserine lactones;
- quorum sensing;
The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl-homoserine lactones (AHLs) and map the resulting AHL profiles onto a host phylogeny.
Methods and Results
We used a high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) protocol to measure AHLs in spent media after bacterial growth. First, the presence/absence of AHLs (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera (Aliivibrio, Photobacterium and Vibrio) of the same family (i.e. Vibrionaceae). Our results show that about half of the isolates produced multiple AHLs, typically at 25–5000 nmol l−1.
This work shows that production of AHL quorum sensing signals is found widespread among Vibrionaceae bacteria and that closely related strains typically produce similar AHL profiles.
Significance and Impact of the Study
The AHL detection protocol presented in this study can be applied to a broad range of bacterial samples and may contribute to a wider mapping of AHL production in bacteria, for example, in clinically relevant strains.