Surface plasmon resonance–based real-time bioaerosol detection
Article first published online: 25 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 3, pages 766–773, September 2013
How to Cite
Usachev, E.V., Usacheva, O.V. and Agranovski, I.E. (2013), Surface plasmon resonance–based real-time bioaerosol detection. Journal of Applied Microbiology, 115: 766–773. doi: 10.1111/jam.12267
- Issue published online: 14 AUG 2013
- Article first published online: 25 JUN 2013
- Accepted manuscript online: 31 MAY 2013 09:51PM EST
- Manuscript Accepted: 28 MAY 2013
- Manuscript Revised: 20 MAY 2013
- Manuscript Received: 21 MAR 2013
- microbial contamination;
- rapid methods
Rapid and precise bioaerosol detection in different environments has become an important research and technological issue over last decades. Previously, we employed a real-time PCR protocol in conjunction with personal bioaerosol sampler for rapid detection of airborne viruses. The approach has been proved to be specific and sensitive. However, a period of time required for entire procedure was in manner of hours. Some new developments are required to decrease the detection time down to real-time protocols.
Methods and Results
Presently, a surface plasmon resonance (SPR)–based immunosensor that coupled with a specific antigen–antibody reaction could offer sensitive, specific, rapid and label-free detection. This study describes the possibility of combining the personal sampler with SPR technology for qualitative and extremely rapid detection of airborne micro-organisms. Common viral surrogate MS2 bacteriophage, frequently used in bioaerosol studies, was employed as a model organism. The results of the sensor functionalizing procedure with monoclonal anti-MS2 antibody and optimization of the chip performance are presented. The SPR-based detection of the airborne virus was found to be very fast; the viral presence was detected in less than 2 min, and the entire procedure (sampling and analysis) was undertaken in 6 min, which could be considered as real-time detection for this type of measurements.
The combination of SPR with the personal sampler targeted towards bioaerosol detection was proven to be feasible. The SPR sensor was found to be highly stable and suitable for multiple utilizations without significant decrease in response. The suggested approach opens new possibilities for the development of portable and rapid (almost real time) bioaerosol monitors.
Significance and Impact of the Study
This technology is the first in the world real-time bioaerosol monitor. This outcome would be of strong interest to individuals representing public health, biosecurity, defence forces, environmental sciences and many others.