Effects of Lactobacillus rhamnosus on asthma with an adoptive transfer of dendritic cells in mice
Article first published online: 24 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 3, pages 872–879, September 2013
How to Cite
Kim, H.-J., Kim, Y.-J., Lee, S.-H., Kang, M.-J., Yu, H.-S., Jung, Y.-H., Lee, E., Seo, J.-H., Kwon, J.-W., Kim, B.-J., Yu, J., Park, H.-M. and Hong, S.-J. (2013), Effects of Lactobacillus rhamnosus on asthma with an adoptive transfer of dendritic cells in mice. Journal of Applied Microbiology, 115: 872–879. doi: 10.1111/jam.12268
- Issue published online: 14 AUG 2013
- Article first published online: 24 JUN 2013
- Accepted manuscript online: 3 JUN 2013 04:40AM EST
- Manuscript Accepted: 24 MAY 2013
- Manuscript Revised: 28 MAR 2013
- Manuscript Received: 26 NOV 2012
- National Research Foundation of Korea (NRF). Grant Number: 2010-0022233
- animal model;
- dendritic cell;
This study was designed to investigate whether the protective effects of Lactobacillus rhamnosus (Lcr35) on allergic asthma are associated with the adoptive transfer of dendritic cells (DCs) and regulatory T cells (Tregs), using a mouse experimental model of asthma.
Methods and Results
BALB/c mice were orally administered Lcr35 or intravenously treated with in vivo Lcr35-treated DCs daily and were then sensitized and challenged with ovalbumin (OVA) in accordance with a model of asthma protocol. Both the oral application of Lcr35 and intravenous administration of Lcr35-treated DCs suppressed all aspects of the asthmatic response, including bronchial hyperresponsiveness (BHR), total cell counts in the bronchoalveolar lavage (BAL) fluid, the production of OVA-specificimmunoglobulin E (IgE), and pulmonary eosinophilic inflammation. The mechanism of action of Lcr35 is related to Tregs, which suppress the Th2 response in the respiratory organs, and this is mediated by DCs in the mouse model of asthma.
These results confirm that the mechanism underlying the effects of Lcr35 on asthma involves the adoptive transfer of DCs.
Significance and Impact of the Study
This finding broadens the possibility that Lcr35 has potential as an alternative therapeutic approach to the treatment of human asthma.