In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase
Article first published online: 24 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 3, pages 880–887, September 2013
How to Cite
Anbazhagan, K., Sasikumar, P., Gomathi, S., Priya, H.P. and Selvam, G.S. (2013), In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase. Journal of Applied Microbiology, 115: 880–887. doi: 10.1111/jam.12269
- Issue published online: 14 AUG 2013
- Article first published online: 24 JUN 2013
- Accepted manuscript online: 4 JUN 2013 10:27AM EST
- Manuscript Revised: 29 MAY 2013
- Manuscript Accepted: 29 MAY 2013
- Manuscript Received: 22 MAR 2013
- oxalate degradation;
- probiotic potential;
- recombinant Lactobacillus plantarum
The aim of the present study is to constitutively express heterologous oxalate decarboxylase (OxdC) in Lactobacillus plantarum and to examine its ability to degrade oxalate in vitro for their future therapy against enteric hyperoxaluria.
Method and Results
In this study, we generated a recombinant strain of Lb. plantarum to constitutively overexpress B. subtilis oxalate decarboxylase (oxdC) using a host lactate dehydrogenase promoter (PldhL). The recombinant Lb. plantarum was able to degrade more than 90% oxalate compared to 15% by the wild type. In addition, the recombinant strain also had higher tolerance up to 500 mmol l−1 oxalate.
We developed a recombinant Lb. plantarum NC8 that constitutively expressed heterologous oxalate decarboxylase and degraded oxalate efficiently under in vitro conditions.
Significance and impact of study
The long-term aim is to develop an efficient strain for future therapy against oxalosis.