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Keywords:

  • hyperoxaluria;
  • oxalate degradation;
  • probiotic potential;
  • recombinant Lactobacillus plantarum

Abstract

Aim

The aim of the present study is to constitutively express heterologous oxalate decarboxylase (OxdC) in Lactobacillus plantarum and to examine its ability to degrade oxalate in vitro for their future therapy against enteric hyperoxaluria.

Method and Results

In this study, we generated a recombinant strain of Lb. plantarum to constitutively overexpress B. subtilis oxalate decarboxylase (oxdC) using a host lactate dehydrogenase promoter (PldhL). The recombinant Lb. plantarum was able to degrade more than 90% oxalate compared to 15% by the wild type. In addition, the recombinant strain also had higher tolerance up to 500 mmol l−1 oxalate.

Conclusion

We developed a recombinant Lb. plantarum NC8 that constitutively expressed heterologous oxalate decarboxylase and degraded oxalate efficiently under in vitro conditions.

Significance and impact of study

The long-term aim is to develop an efficient strain for future therapy against oxalosis.