Adherence and biofilm formation of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium abscessus to household plumbing materials
Article first published online: 24 JUN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 3, pages 908–914, September 2013
How to Cite
Mullis, S.N. and Falkinham, J.O. (2013), Adherence and biofilm formation of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium abscessus to household plumbing materials. Journal of Applied Microbiology, 115: 908–914. doi: 10.1111/jam.12272
- Issue published online: 14 AUG 2013
- Article first published online: 24 JUN 2013
- Accepted manuscript online: 6 JUN 2013 09:41AM EST
- Manuscript Accepted: 31 MAY 2013
- Manuscript Revised: 20 MAY 2013
- Manuscript Received: 5 MAR 2013
- nontuberculous mycobacteria;
- pipe surfaces
Measure adherence and biofilm formation by cells of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium abscessus on common household plumbing materials namely stainless steel, glass, zinc-galvanized steel, copper and polyvinyl chloride (PVC).
Methods and Results
Coupons in a CDC biofilm reactor were exposed to cell suspensions containing 105 NTM colony forming units (CFU) per ml and adherence measured for 6 h. Biofilm formation (increased numbers of adherent CFU) was measured weekly to 21 days in the absence of substantial numbers of suspended mycobacterial cells. Adherence was rapid and substantial with 2000–15 000 CFU cm−2 adhering within 1–6 h at room temperature. Biofilm numbers reached as high as 107 CFU cm−2. Biofilm-grown cells of Myco. avium were more adherent compared with suspension-grown cells.
Mycobacterium avium, Myco. intracellulare and Myco. abscessus readily adhered and formed biofilms on all types of plumbing materials. Factors influencing adherence and biofilm formation were species, plumbing material and prior growth.