Temperature and respiration affect the growth and stress resistance of Lactobacillus plantarum C17
Article first published online: 19 JUL 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 3, pages 848–858, September 2013
How to Cite
Zotta, T., Guidone, A., Ianniello, R.G., Parente, E. and Ricciardi, A. (2013), Temperature and respiration affect the growth and stress resistance of Lactobacillus plantarum C17. Journal of Applied Microbiology, 115: 848–858. doi: 10.1111/jam.12285
- Issue published online: 14 AUG 2013
- Article first published online: 19 JUL 2013
- Accepted manuscript online: 19 JUN 2013 09:00AM EST
- Manuscript Accepted: 14 JUN 2013
- Manuscript Revised: 10 MAY 2013
- Manuscript Received: 8 FEB 2013
- Ministero dell'Istruzione
- dell'Università e della Ricerca. Grant Number: 359 20088SZB9B
- aerobic enzymes;
- Lactobacillus plantarum ;
- stress response
The aim of the study is to gain further insight on the respiratory behaviour of Lactobacillus plantarum and its consequences on stress tolerance.
Methods and Results
We investigated the effect of temperature and respiration on the growth and stress (heat, oxidative, freezing, freeze-drying) response of Lact. plantarum C17 during batch cultivations. Temperature as well as respiration clearly affected the physiological state of cells, and generally, cultures grown under respiratory conditions exhibited improved tolerance of some stresses (heat, oxidative, freezing) compared to those obtained in anaerobiosis. Our results revealed that the activities in cell-free extracts of the main enzymes related to aerobic metabolism, POX (pyruvate oxidase) and NPR (NADH peroxidase), were significantly affected by temperature. POX was completely inhibited at 37°C, while the activity of NPR slightly increased at 25°C, indicating that in Lact. plantarum, the temperature of growth may be involved in the activation and modulation of aerobic/respiratory metabolism.
We confirmed that respiration confers robustness to Lact. plantarum cells, allowing a greater stress tolerance and advantages in the production of starter and probiotic cultures.
Significance and Impact of the Study
This is the first study on respiratory metabolism on a strain other than the model strains WCFS1; novel information on the role of temperature in the modulation of aerobic/respiratory metabolism in Lact. plantarum is presented.