Inhibition of initial adhesion of oral bacteria through a lectin from Bauhinia variegata L. var. variegata expressed in Escherichia coli
Article first published online: 3 SEP 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 5, pages 1222–1230, November 2013
How to Cite
Klafke, G.B., Borsuk, S., Gonçales, R.A., Arruda, F.V.S., Carneiro, V.A., Teixeira, E.H., Coelho da Silva, A.L., Cavada, B.S., Dellagostin, O.A. and Pinto, L.S. (2013), Inhibition of initial adhesion of oral bacteria through a lectin from Bauhinia variegata L. var. variegata expressed in Escherichia coli. Journal of Applied Microbiology, 115: 1222–1230. doi: 10.1111/jam.12318
- Issue published online: 15 OCT 2013
- Article first published online: 3 SEP 2013
- Accepted manuscript online: 3 AUG 2013 01:07AM EST
- Manuscript Accepted: 27 JUL 2013
- Manuscript Revised: 24 JUN 2013
- Manuscript Received: 24 APR 2013
- CAPES Foundation. Grant Numbers: 19120/2009-3, 02841/09-6
- Fundação de Amparo a Pesquisa do Rio Grande do Sul. Grant Number: 11/1842-5
- acquired pellicle;
- Streptococcus mutans ;
- Streptococcus sanguis ;
- Streptococcus sobrinus
The aim of the present work was to study the in vitro effect of native and recombinant Bauhinia variegata var. variegata lectins in inhibiting early adhesion of Streptococcus mutans, Streptococcus sanguis and Streptococcus sobrinus to experimentally acquired pellicle.
Methods and Results
Native lectin from B. variegata (BVL) was purified by affinity chromatography of extract of seeds. The recombinant lectin (rBVL-I) was expressed in E. coli strain BL21 (DE3) from a genomic clone encoding the mature B. variegata lectin gene using the vector pAE-bvlI. Recombinant protein deposited in inclusion bodies was solubilized and subsequently purified by affinity chromatography. The rBVL-I was compared to BVL for agglutination of erythrocytes and initial adherence of oral bacteria on a saliva-coated surface. The results revealed that rBVL-I acts similarly to BVL for agglutination of erythrocytes. Both lectins showed adhesion inhibition effect on Step. sanguis, Step. mutans and Step. sobrinus.
We report, for the first time, the inhibition of early adhesion of oral bacteria by a recombinant lectin.
Significance and Impact of the Study
Our results support the proposed biotechnological application of lectins in a strategy to reduce development of dental caries by inhibiting the initial adhesion and biofilm formation.