These authors contributed equally.
D-cycloserine or similar physiochemical compounds may be uniquely suited for use in Bacillus anthracis spore decontamination strategies
Article first published online: 30 AUG 2013
© 2013. This article is a U.S. Government work and is in the public domain in the USA
Journal of Applied Microbiology
Volume 115, Issue 6, pages 1343–1356, December 2013
How to Cite
Omotade, T.O., Heffron, J.D., Klimko, C.P., Marchand, C.L., Miller, L.L., Halasahoris, S.A., Bozue, J.A., Welkos, S.L. and Cote, C.K. (2013), D-cycloserine or similar physiochemical compounds may be uniquely suited for use in Bacillus anthracis spore decontamination strategies. Journal of Applied Microbiology, 115: 1343–1356. doi: 10.1111/jam.12322
- Issue published online: 15 NOV 2013
- Article first published online: 30 AUG 2013
- Accepted manuscript online: 8 AUG 2013 04:56AM EST
- Manuscript Accepted: 30 JUL 2013
- Manuscript Revised: 18 JUL 2013
- Manuscript Received: 5 JUN 2013
- Defense Threat Reduction Agency JSTO-CBD plan. Grant Number: CBT-PHM-12-CB2-CB3831
- Bacillus anthracis ;
As observed in the aftermath of the anthrax attacks of 2001, decontamination and remediation of a site contaminated by the accidental or intentional release of Bacillus anthracis spores is difficult, costly and potentially damaging to the environment. The identification of novel strategies that neutralize the threat of spores while minimizing environmental damage remains a high priority. We investigated the efficacy of d-cycloserine (DCS), an antibiotic and inhibitor of the spore-associated enzyme (alanine racemase) responsible for converting l-alanine to d-alanine, as a spore germination enhancer and antimicrobial agent.
Methods and Results
We characterized the impact of DCS exposure on both germinating spores and vegetative cells of fully virulent B. anthracis by evaluating spore germination kinetics, determining the minimum inhibitory concentrations (MICs) required to affect growth of the bacteria and performing macrophage viability assays. DCS enhanced germination induced by l-alanine and also efficiently killed the newly germinated spores. Furthermore, DCS proved nontoxic to macrophages at concentrations that provided protection from the killing effects of spores. Similar tests were conducted with Bacillus thuringiensis (subspecies kurstaki and Al Hakam) to determine its potential as a possible surrogate for B. anthracis field trials. Bacillus thuringiensis spores responded in a similar manner to B. anthracis spores when exposed to DCS.
These results further support that DCS augments the germination response of spores in the presence of l-alanine but also reveal that DCS is bactericidal towards germinating spores.
Significance and Impact of the Study
DCS (or similar compounds) may be uniquely suited for use as part of decontamination strategies by augmenting the induction of spore germination and then rendering the germinated spores nonviable.