Immune responses of chickens inoculated with recombinant Lactobacillus expressing the haemagglutinin of the avian influenza virus
Article first published online: 5 SEP 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 6, pages 1269–1277, December 2013
How to Cite
Wang, Z., Yu, Q., Fu, J., Liang, J. and Yang, Q. (2013), Immune responses of chickens inoculated with recombinant Lactobacillus expressing the haemagglutinin of the avian influenza virus. Journal of Applied Microbiology, 115: 1269–1277. doi: 10.1111/jam.12325
- Issue published online: 15 NOV 2013
- Article first published online: 5 SEP 2013
- Accepted manuscript online: 12 AUG 2013 08:00AM EST
- Manuscript Accepted: 5 AUG 2013
- Manuscript Revised: 17 JUL 2013
- Manuscript Received: 30 MAR 2013
- National Science Grant of China. Grant Number: 31172302
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- avian influenza virus;
- Lactobacillus ;
- mucosal immune
To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17-pH) that expresses the foreign HPAI protein, haemagglutinin 1 (HA1).
Methods and Results
The mucosal and systemic immune responses that are triggered by LDL17-pH following the oral administration to 10-day-old chickens were evaluated. The results showed that LDL17-pH could significantly increase the specific anti-HA1 IgA antibody level in the mucosa and the anti-HA1 IgG level in sera. Tissues were isolated from trachea and Peyer's patches(PPs)and caecal tonsils of chickens, and gene expression was analysed via real-time quantitative PCR.
The results showed that LDL17-pH could significantly induce the specific anti-HA1 IgA antibody level in the trachea and intestine and the specific anti-HA1 IgG antibody level in the serum (P < 0·05). Additionally, LDL17-pH was in the capacity to induce the expression of cytokines IFN-γ, TLR-2 and AvBD-9 in the PPs and caecal tonsils. Most importantly, the chickens that were immunized with LDL17-pH were protected against lethal challenge of the H5N1 virus to some extent.
Significance and Impact of the Study
Therefore, LDL17-pH could be a promising oral vaccine candidate against HPAI.