Evolution of pyruvate kinase-deficient Escherichia coli mutants enables glycerol-based cell growth and succinate production
Article first published online: 6 SEP 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 6, pages 1368–1378, December 2013
How to Cite
Soellner, S., Rahnert, M., Siemann-Herzberg, M., Takors, R. and Altenbuchner, J. (2013), Evolution of pyruvate kinase-deficient Escherichia coli mutants enables glycerol-based cell growth and succinate production. Journal of Applied Microbiology, 115: 1368–1378. doi: 10.1111/jam.12333
- Issue published online: 15 NOV 2013
- Article first published online: 6 SEP 2013
- Accepted manuscript online: 20 AUG 2013 12:31AM EST
- Manuscript Accepted: 13 AUG 2013
- Manuscript Revised: 9 AUG 2013
- Manuscript Received: 26 MAR 2013
- Stiftung Baden-Württemberg
Table S1 Detailed pedigree of E. coli strains.
Table S2 Oligonucleotides used for constructing gene deletion cassettes.
Table S3 Primers used for cloning or sequencing.
Table S4 Oligonucleotides for strain and deletion verification.
Table S5 Verification of the gldA deletion by genomic PCR and following restriction of products.
Table S6 Requirement of pyruvate for growth of ss173, ss195 and ss251 on various PTS and non-PTS sugars.
Table S7 Mutations identified in E. coli ss251.
Figure S1 Analysis of the pykA, pykF and tdcE deletions/gene replacements in the strains ss195, ss251, ss279 and comparison with the wild-type ss173.
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