Investigation of potential markers of acid resistance in Lactobacillus plantarum by comparative proteomics
Article first published online: 30 SEP 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 1, pages 134–144, January 2014
How to Cite
Hamon, E., Horvatovich, P., Marchioni, E., Aoudé-Werner, D. and Ennahar, S. (2014), Investigation of potential markers of acid resistance in Lactobacillus plantarum by comparative proteomics. Journal of Applied Microbiology, 116: 134–144. doi: 10.1111/jam.12339
- Issue published online: 16 DEC 2013
- Article first published online: 30 SEP 2013
- Accepted manuscript online: 10 SEP 2013 02:20AM EST
- Manuscript Revised: 3 SEP 2013
- Manuscript Accepted: 3 SEP 2013
- Manuscript Received: 29 MAY 2013
- ‘Ministère de l'Enseignement Supérieur et de la Recherche’
- “Ministère de l'Agriculture et de la Pêche”
- acid resistance;
- comparative proteomics;
- Lactobacillus plantarum ;
To investigate cell determinants of resistance to gastric acidity in Lactobacillus plantarum using comparative proteomics.
Methods and Results
Among ten Lact. plantarum strains that were tested for their acid resistance in vitro, three strains with different phenotypes were selected for comparative proteomic analysis: LC 804 (resistant), CIP A159 (intermediate) and CECT 4185 (sensitive). Constitutive differences between whole-cell proteomes of the three strains were studied. Among the differentially expressed proteins between strains, 17 have previously been reported to be involved in acid resistance processes. The effect of a low-pH exposure on these proteomic patterns was investigated, which led to the identification of five putative determinants of acid resistance (heat-shock protein GrpE, methionine synthase and 30S ribosomal protein S2) or sensitivity (phosphotransacetylase and adenylosuccinate synthase). Analysis also revealed three additional proteins involved in cell envelope biogenesis (3-oxoacyl-synthase II, dTDP-glucose 4,6-dehydratase and dTDP-4-dehydrorhamnose 3,5-epimerase) likely to be key factors of intrinsic acid tolerance in Lact. plantarum.
The approach used in this study enabled the identification of potential markers of acid tolerance in Lact. plantarum, which may serve for phenotyping and screening purposes.
Significance and Impact of the Study
The present work represents a further step towards the identification of bacterial biomarkers for each particular probiotic feature.