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Investigation of potential markers of acid resistance in Lactobacillus plantarum by comparative proteomics

Authors

  • E. Hamon,

    1. Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Illkirch-Graffenstaden, France
    2. Aérial Parc d'Innovation, Illkirch-Graffenstaden, France
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  • P. Horvatovich,

    1. Department of Analytical Biochemistry, Centre for Pharmacy, University of Groningen, Groningen, the Netherlands
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  • E. Marchioni,

    1. Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Illkirch-Graffenstaden, France
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  • D. Aoudé-Werner,

    1. Aérial Parc d'Innovation, Illkirch-Graffenstaden, France
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  • S. Ennahar

    Corresponding author
    1. Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Illkirch-Graffenstaden, France
    • Correspondence

      Saïd Ennahar, Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, UMR7178 CNRS, 74 route du Rhin, 67400 Illkirch-Graffenstaden, France. E-mail: ennahar@unistra.fr

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Abstract

Aims

To investigate cell determinants of resistance to gastric acidity in Lactobacillus plantarum using comparative proteomics.

Methods and Results

Among ten Lact. plantarum strains that were tested for their acid resistance in vitro, three strains with different phenotypes were selected for comparative proteomic analysis: LC 804 (resistant), CIP A159 (intermediate) and CECT 4185 (sensitive). Constitutive differences between whole-cell proteomes of the three strains were studied. Among the differentially expressed proteins between strains, 17 have previously been reported to be involved in acid resistance processes. The effect of a low-pH exposure on these proteomic patterns was investigated, which led to the identification of five putative determinants of acid resistance (heat-shock protein GrpE, methionine synthase and 30S ribosomal protein S2) or sensitivity (phosphotransacetylase and adenylosuccinate synthase). Analysis also revealed three additional proteins involved in cell envelope biogenesis (3-oxoacyl-synthase II, dTDP-glucose 4,6-dehydratase and dTDP-4-dehydrorhamnose 3,5-epimerase) likely to be key factors of intrinsic acid tolerance in Lact. plantarum.

Conclusions

The approach used in this study enabled the identification of potential markers of acid tolerance in Lact. plantarum, which may serve for phenotyping and screening purposes.

Significance and Impact of the Study

The present work represents a further step towards the identification of bacterial biomarkers for each particular probiotic feature.

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