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Identification of dichloroacetic acid degrading Cupriavidus bacteria in a drinking water distribution network model

Authors

  • C. Berthiaume,

    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
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    • These authors have equal contribution.
  • Y. Gilbert,

    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
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    • These authors have equal contribution.
  • J. Fournier-Larente,

    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
    2. Institut de biologie intégrative et des systèmes, Université Laval, Quebec City, QC, Canada
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  • C. Pluchon,

    1. Département de génie civil et de génie des eaux, Faculté des sciences et de génie, Université Laval, Quebec City, QC, Canada
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  • G. Filion,

    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
    2. Institut de biologie intégrative et des systèmes, Université Laval, Quebec City, QC, Canada
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  • E. Jubinville,

    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
    2. Institut de biologie intégrative et des systèmes, Université Laval, Quebec City, QC, Canada
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  • J.-B. Sérodes,

    1. Département de génie civil et de génie des eaux, Faculté des sciences et de génie, Université Laval, Quebec City, QC, Canada
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  • M. Rodriguez,

    1. Centre de recherche en aménagement et en développement, Pavillon Félix-Antoine-Savard, Université Laval, Quebec City, QC, Canada
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  • C. Duchaine,

    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
    2. Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Quebec City, QC, Canada
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  • S.J. Charette

    Corresponding author
    1. Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Quebec City, QC, Canada
    2. Institut de biologie intégrative et des systèmes, Université Laval, Quebec City, QC, Canada
    3. Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Quebec City, QC, Canada
    • Correspondence

      Steve J. Charette, Institut de biologie intégrative et des systèmes, Université Laval, 1030 avenue de la Médecine, Quebec City, QC, Canada G1V 0A6.

      E-mail: steve.charette@bcm.ulaval.ca

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Abstract

Aims

Bacterial community structure and composition of a drinking water network were assessed to better understand this ecosystem in relation to haloacetic acid (HAA) degradation and to identify new bacterial species having HAA degradation capacities.

Methods and Results

Biofilm samples were collected from a model system, simulating the end of the drinking water distribution network and supplied with different concentrations of dichloroacetic and trichloroacetic acids at different periods over the course of a year. The samples were analysed by culturing, denaturing gradient gel electrophoresis (DGGE) and sequencing. Pipe diameter and HAA ratios did not impact the bacterial community profiles, but the season had a clear influence. Based on DGGE profiles, it appeared that a particular biomass has developed during the summer compared with the other seasons. Among the bacteria isolated in this study, those from genus Cupriavidus were able to degrade dichloroacetic acid. Moreover, these bacteria degrade dichloroacetic acid at 18°C but not at 10°C.

Conclusions

The microbial diversity evolved throughout the experiment, but the bacterial community was distinct during the summer. Results obtained on the capacity of Cupriavidus to degrade DCAA only at 18°C but not at 10°C indicate that water temperature is a major element affecting DCAA degradation and confirming observations made regarding season influence on HAA degradation in the drinking water distribution network.

Significance and Impact of the Study

This is the first demonstration of the HAA biodegradation capacity of the genus Cupriavidus.

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