Fluorescence in situ hybridization probing of protozoal Entodinium spp. and their methanogenic colonizers in the rumen of cattle fed alfalfa hay or triticale straw
Article first published online: 24 OCT 2013
Her Majesty the Queen in Right of Canada © 2013 Society for Applied Microbiology Reproduced with the permission of the Minister of Agriculture and Agri-Food Canada.
Journal of Applied Microbiology
Volume 116, Issue 1, pages 14–22, January 2014
How to Cite
Xia, Y., Kong, Y.H., Seviour, R., Forster, R.J., Kisidayova, S. and McAllister, T.A. (2014), Fluorescence in situ hybridization probing of protozoal Entodinium spp. and their methanogenic colonizers in the rumen of cattle fed alfalfa hay or triticale straw. Journal of Applied Microbiology, 116: 14–22. doi: 10.1111/jam.12356
- Issue published online: 16 DEC 2013
- Article first published online: 24 OCT 2013
- Accepted manuscript online: 1 OCT 2013 10:50AM EST
- Manuscript Accepted: 23 SEP 2013
- Manuscript Revised: 12 JUL 2013
- Manuscript Received: 8 FEB 2013
- Entodinium ;
- fluorescence in situ hybridization;
- rumen ecology;
To develop and test a fluorescence in situ hybridization (FISH) based technique and to identify and quantify simultaneously those methanogenic populations colonizing Entodinium spp. in the rumen of cows fed different forages.
Methods and Results
New FISH probes targeting protozoal Entodinium spp. were designed and used together with FISH probes for methanogens in the cow rumen. The composition and relative abundance of methanogenic populations colonizing Entodinium simplex-, E. caudaum- and Entodinium furca-related populations were similar. Methanogens including Methanobrevibacter thaueri, Methanobrevibacter millerae and Methanobrevibacter smithii, and members of Methanomicrobium and Methanosphaera were generally the predominant colonizers of protozoa, regardless of the forage fed to cattle. Individual animals appeared to differ in which ruminal methanogenic populations colonized each of the individual Entodinium spp.
Simultaneous FISH probing is shown here to be a reliable and effective approach to investigate the dynamics of symbiotic relationships between ruminal protozoa and methanogens at a single cell level. Phylogenetically closely related Entodinium spp. were colonized by similar methanogenic populations regardless of the forage fed.
Significance and Impact of the Study
This is the first report of the methanogenic archaeal populations that specifically colonize Entodinium spp. as identified using simultaneous FISH probing.