Hydrolysates of glycated and heat-treated peanut 7S globulin (Ara h 1) modulate human gut microbial proliferation, survival and adhesion

Authors

  • M. Teodorowicz,

    1. Faculty of Biology, University of Warmia and Mazury, Olsztyn, Poland
    2. Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands
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    • These two authors contributed equally to this study.

  • D. Świątecka,

    Corresponding author
    1. Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
    • Correspondence

      Dominika Świątecka, Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10 str, 10-748 Olsztyn, Poland.

      E-mail: d.swiatecka@pan.olsztyn.pl

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    • These two authors contributed equally to this study.

  • H. Savelkoul,

    1. Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • H. Wichers,

    1. Food and Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • E. Kostyra

    1. Faculty of Biology, University of Warmia and Mazury, Olsztyn, Poland
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Abstract

Aims

Evaluation of an effect of glycation of Ara h 1 on proliferation and survival rate and adhesion of intestinal Enterococcus faecalis, Escherichia coli and Lactobacillus acidophilus.

Methods and Results

Pure Ara h 1 heated at three different temperature conditions (G37, G60 and C145°C) in the presence or absence of glucose was subjected to enzymatic hydrolysis. Impacts of Ara h 1 hydrolysates on the bacterial proliferation, survival rate and adhesion to Caco-2 cells in mono and heterogeneous cultures were studied with fluorescent techniques: DAPI, LIVE/DEAD staining and FISH. Examined hydrolysates hindered proliferation of E. coli and Ent. faecalis with simultaneous decrease in their survival. Maillard reaction (MR, glycation) of Ara h 1 did not alter the effect of hydrolysates on bacterial proliferation rate. Hydrolysates modified at 60 and 145°C with glucose altered the profile of immobilized bacteria, mostly by lowering the number of adhering E. coli and promoting the adhesion of bacteria from genera Lactobacillus and Enterococcus.

Conclusions

Ara h1 hydrolysates processed in various ways demonstrated their strong modulatory effect on bacterial proliferation, survival rate and adhesion.

Significance and Impact of the Study

Reducing the adhesion of opportunistic bacteria by hydrolysates of Ara h 1 glycated at 60 and 145°C, together with modulation of immobilization of beneficial lactobacilli and enterococci, may be of relevance in terms of the physiological status of the intestinal barrier.

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