Brevibacillus themoruber: a promising microbial cell factory for exopolysaccharide production
Article first published online: 30 OCT 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 2, pages 314–324, February 2014
How to Cite
Yasar Yildiz, S., Anzelmo, G., Ozer, T., Radchenkova, N., Genc, S., Di Donato, P., Nicolaus, B., Toksoy Oner, E. and Kambourova, M. (2014), Brevibacillus themoruber: a promising microbial cell factory for exopolysaccharide production. Journal of Applied Microbiology, 116: 314–324. doi: 10.1111/jam.12362
- Issue published online: 17 JAN 2014
- Article first published online: 30 OCT 2013
- Accepted manuscript online: 7 OCT 2013 03:06AM EST
- Manuscript Accepted: 1 OCT 2013
- Manuscript Revised: 18 SEP 2013
- Manuscript Received: 2 JUL 2013
- The Scientific and Technological Research Council of Turkey (TUBITAK). Grant Numbers: BIDEB/2221, TBAG/111T016
- Marmara University. Grant Number: FEN-C-YLP-210311-0057
- Brevibacillus thermoruber ;
This study aims to identify a high level exopolysaccharide (EPS) producer thermophile that in turn could be used as a model organism to study the biological mechanisms and whole genome organization of EPS-producing thermophilic bacteria.
Methods and Results
Thermophilic isolates were screened, and then growth and EPS production of the best producer Brevibacillus thermoruber strain 423 were investigated under different carbon and nitrogen sources, temperature, pH and agitation rates. Rheological characterization revealed that the EPS behaved like a typical Newtonian fluid and viscosity of the EPS solution increased with increasing Ca2+ ion concentration. Chemical characterization by TLC, GC-MS, FT-IR and NMR suggested a heteropolymer structure with glucose as major monomer unit. High biocompatibility of pure EPS fractions suggested their potential use in biomedical applications.
This study reports on the comprehensive description of microbial production conditions as well as chemical, rheological and biological characterization of the EPS produced by B. thermoruber strain 423. The bioreactor cultures were found to reach two times higher yields and three times higher productivities when compared with literature.
Significance and Impact of the Study
Brevibacillus thermoruber strain 423 combined the advantages of its nonpathogenicity with the advantages of fast productivity and hence proved to be a very promising model organism and cell factory for microbial EPS production.