Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong
Article first published online: 5 NOV 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 2, pages 464–476, February 2014
How to Cite
Sun, W., Xu, M.-Y., Wu, W.-M., Guo, J., Xia, C.-Y., Sun, G.-P. and Wang, A.-J. (2014), Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong. Journal of Applied Microbiology, 116: 464–476. doi: 10.1111/jam.12367
- Issue published online: 17 JAN 2014
- Article first published online: 5 NOV 2013
- Accepted manuscript online: 11 OCT 2013 10:58AM EST
- Manuscript Accepted: 8 OCT 2013
- Manuscript Revised: 21 SEP 2013
- Manuscript Received: 16 JUN 2013
- National Natural Science Foundation of China. Grant Number: 31170470
- National Basic Research Program of China. Grant Number: 2012CB22307
- Chinese National Programs for High Technology Research and Development. Grant Number: 2011AA060904
- Teamwork Project of the Natural Science Foundation of Guangdong Province, China. Grant Number: 9351007002000001
- Guangdong Province – Chinese Academy of Sciences. Grant Number: 2010B090301048
- river sediment;
- the Dongjiang River;
- 16S rRNA gene;
- hzo gene
The aim of this study was to characterize anaerobic ammonium oxidation (anammox) community in sediments of the Dongjiang River, a drinking water source of Hong Kong.
Methods and Results
The diversity and distribution of the anammox community were investigated based on a comparative analyses of 16S rRNA and hydrazine oxidation (hzo) genes of anammox bacteria. Candidatus Brocadia and two new anammox bacterial clusters were detected based on phylogenetic analysis of 16S rRNA genes. In contrast, the targeting of hzo genes indicated the presence of only Candidatus Jettenia with four different clusters. It was found that the sequence diversities of hzo genes were higher than those of the 16S rRNA genes. The abundance of anammox bacteria varied significantly among the sediment samples based on qPCR. Pearson correlation analysis indicated that nitrite concentration was the key factor influencing the abundance of anammox bacteria. The redundance analysis (RDA) confirmed that the combination of the contents of nitrite and nitrate, and the ratio of total nitrogen vs total carbon (TN/TC) had significant impact on the anammox bacterial community structure.
The results revealed that the diverse anammox bacteria were present in sediments of the Dongjiang River, and the community structures were associated with varied environmental factors caused by urban pollutant invasion.
Significance and Impact of the Study
This is the first report about the distribution of anammox bacterial community in sediments of the Dongjiang River, which provides helpful information of anammox niche specificity and influencing factors in the river ecosystem.