Dynamics of Neotyphodium uncinatum and N-formylloline in Italian ryegrass, and their relation to insect resistance in the field
Article first published online: 13 NOV 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
How to Cite
Matsukura, K., Shiba, T., Sasaki, T., Yoshida, K. and Matsumura, M. (2013), Dynamics of Neotyphodium uncinatum and N-formylloline in Italian ryegrass, and their relation to insect resistance in the field. Journal of Applied Microbiology. doi: 10.1111/jam.12374
- Article first published online: 13 NOV 2013
- Accepted manuscript online: 25 OCT 2013 03:14AM EST
- Manuscript Accepted: 21 OCT 2013
- Manuscript Revised: 15 OCT 2013
- Manuscript Received: 14 JUN 2013
- Science and Technology Research promotion program for agriculture, forestry, fisheries and food industry. Grant Number: 25024A
- Ministry of Agriculture, Forestry, and Fisheries of Japan
- crop protection;
- forage grass;
- Laodelphax striatellus ;
- Stenotus rubrovittatus
A fungal endophyte, Neotyphodium uncinatum, accumulates N-formylloline, which is toxic to Hemipteran insects, in Italian ryegrass. This study aimed to clarify the dynamics of N. uncinatum and N-formylloline in Italian ryegrass, and their relationship to insect resistance.
Methods and Results
Changes in the density and localization of N. uncinatum and N-formylloline in N. uncinatum-infected Italian ryegrass were examined by real-time PCR and gas chromatography, respectively. Neotyphodium uncinatum multiplied on pseudostems at the flowering stage, and then increased on inflorescences at the ripening stage. On the other hand, N-formylloline accumulated heavily in inflorescences and leaf blades, but lightly in pseudostems at the ripening stage. In field experiments, N. uncinatum-infected Italian ryegrass suppressed the occurrence of Stenotus rubrovittatus, which fed on the inflorescences, but was not effective to Laodelphax striatellus, which do not necessarily prefer a particular plant tissue.
Localization of N. uncinatum and N-formylloline were discordant in Italian ryegrass. The N. uncinatum-infected Italian ryegrass was effective to only insects that prefer to feed on particular plant tissues containing N-formylloline.
Significance and Impact of Study
Our data implies that the relationship between insect habits and the dynamics of alkaloids in plants is important for the effective use of endophyte-infected crops.