Effects of Lactobacillus acidophilus and inulin on faecal viral shedding and immunization against H9N2 Avian influenza virus
Article first published online: 27 NOV 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 3, pages 667–676, March 2014
How to Cite
Poorbaghi, S.L., Dadras, H., Gheisari, H.R., Mosleh, N., Firouzi, S. and Roohallazadeh, H. (2014), Effects of Lactobacillus acidophilus and inulin on faecal viral shedding and immunization against H9N2 Avian influenza virus. Journal of Applied Microbiology, 116: 667–676. doi: 10.1111/jam.12390
- Issue published online: 14 FEB 2014
- Article first published online: 27 NOV 2013
- Accepted manuscript online: 11 NOV 2013 04:50AM EST
- Manuscript Accepted: 7 NOV 2013
- Manuscript Revised: 12 OCT 2013
- Manuscript Received: 30 JUL 2013
- Shiraz University Research Council. Grant Number: 91GCU1M1756
- avian influenza;
- Lactobacillus acidophilus ;
The aims of this investigation were to compare the effects of Lactobacillus acidophilus addition as simple or microencapsulated (ME) probiotic and inulin as prebiotic to the broiler diet on the faecal viral shedding and immunization against avian influenza virus (AIV) with or without H9N2 vaccination.
Methods and Results
Simple or ME forms of Lact. acidophilus, inulin and combination of them as synbiotic were analysed for their ability to enhance immunity against H9N2 AIV and to decrease faecal viral shedding in Cobb-500 broiler chicks. Our results indicated that probiotic as ME form can decrease haemagglutination inhibition (HI) titre significantly on days 34 in vaccinated trial (P < 0·05). Also, the effects of ME form of probiotic are more remarkable on reduction of viral faecal shedding detected by RT-PCR.
The study shows the significant role of microencapsulation on probiotic effects against H9N2 AIV.
Significance and Impact of the Study
The application of probiotics especially in the ME form could have the potential for stimulating the immune system, preventing influenza infection and consequently reduce faecal viral shedding of H9N2 AIV.