Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample
Article first published online: 3 DEC 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 3, pages 502–510, March 2014
How to Cite
Berić, T., Stanković, S., Draganić, V., Kojić, M., Lozo, J. and Fira, D. (2014), Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample. Journal of Applied Microbiology, 116: 502–510. doi: 10.1111/jam.12393
- Issue published online: 14 FEB 2014
- Article first published online: 3 DEC 2013
- Accepted manuscript online: 17 NOV 2013 11:20PM EST
- Manuscript Revised: 8 NOV 2013
- Manuscript Accepted: 8 NOV 2013
- Manuscript Received: 18 SEP 2013
- Ministry of Education, Science and Technological Development. Grant Number: 173026
- Bacillus licheniformis ;
- licheniocin 50.2;
- subclass II.3
To isolate and characterize bacteriocin, licheniocin 50.2, from soil bacteria identified as Bacillus licheniformis.
Methods and Results
The strain B. licheniformis VPS50.2 was identified as bacteriocin producer, effective against Gram-positive bacteria, including Listeria monocytogenes, methicillin-resistant Staphylococcus aureus (MRSA) and β-haemolytic streptococci. The start of bacteriocin production coincides with the beginning of sporulation. Ammonium sulfate precipitation, chloroform extraction and ultrafiltration were used for bacteriocin purification. MALDI TOF/TOF mass spectrometry of purified sample detected the protein with molecular mass of 3253·209 Da. N-terminal sequencing recognized first 15 amino acids with the sequence: W E E Y N I I X Q L G N K G Q. We named the newly characterized bacteriocin as subclass II.3 bacteriocin, licheniocin 50·2. The bacteriocin activity was insensitive to lysozyme and proteinase K, heat stable after incubation at 100°C for 30 min and over wide range of pH (2–12). MICs of crude bacteriocin extract were determined for L. monocytogenes and MRSA. Time–kill study showed that licheniocin had bactericidal effect to L. monocytogenes.
A novel, thermostable, pH-tolerant bacteriocin active against Gram-positive bacteria was isolated.
Significance and Impact of the Study
Attributes of new, stable licheniocin 50.2 make it a promising agent for application as biopreservative in food industry.