Characterization of the response to low pH of Lactobacillus casei ΔRR12, a mutant strain with low D-alanylation activity and sensitivity to low pH
Article first published online: 8 FEB 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 5, pages 1250–1261, May 2014
How to Cite
Revilla-Guarinos, A., Alcántara, C., Rozès, N., Voigt, B. and Zúñiga, M. (2014), Characterization of the response to low pH of Lactobacillus casei ΔRR12, a mutant strain with low D-alanylation activity and sensitivity to low pH. Journal of Applied Microbiology, 116: 1250–1261. doi: 10.1111/jam.12442
- Issue published online: 11 APR 2014
- Article first published online: 8 FEB 2014
- Accepted manuscript online: 18 JAN 2014 05:23AM EST
- Manuscript Accepted: 6 JAN 2014
- Manuscript Revised: 8 NOV 2013
- Manuscript Received: 2 AUG 2013
- Spanish Ministry of Science and Innovation. Grant Numbers: AGL2007-60975, AGL2010-15679
- Generalitat Valenciana. Grant Number: ACOMP2012/137
- acid sensitivity;
- acid tolerance response;
- D-alanylation of teichoic acids;
- fatty acids;
- Lactobacillus casei
To identify the differences that account for the acid sensitivity of Lactobacillus casei ΔRR12. RR12 controls the expression of the dlt operon, and its inactivation leads to a diminished teichoic acid D-alanylation activity. To this end, a comparison of its response of ΔRR12 to low pH with the parental strain Lact. casei BL23 was carried out.
Methods and Results
The ability to induce an acid tolerance response (ATR), fatty acid (FA) composition and proteome changes induced in both strains in response to acid were investigated. Results obtained showed that both strains induce a growth-phase-dependent ATR. However, significant differences in the content of FAs and membrane-associated proteins were detected.
The greater abundance of cytoplasmic proteins in the membrane fraction of the mutant strain ΔRR12 suggests an increased permeability of the cell membrane in this strain.
Significance and Impact of the Study
The analysis of the response to low pH of strain ΔRR12 indicated that the inactivation of TCS12 affected the content of FAs and proteins associated to the cell envelope. Increased abundance of cytoplasmic proteins suggested that low alanylation of teichoic acids affected the permeability of the cell membrane and possibly accounts for the acid sensitivity of strain ΔRR12.