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Membrane disruption and anti-quorum sensing effects of synergistic interaction between Lavandula angustifolia (lavender oil) in combination with antibiotic against plasmid-conferred multi-drug-resistant Escherichia coli

Authors

  • P.S.X. Yap,

    1. School of Postgraduate Studies and Research, International Medical University, Kuala Lumpur, Malaysia
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  • T. Krishnan,

    1. Faculty of Science, Division of Genetics and Molecular Biology, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
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  • B.C. Yiap,

    1. Department of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia
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  • C.P. Hu,

    1. Department of Chinese Medicine, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia
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  • K.-G. Chan,

    1. Faculty of Science, Division of Genetics and Molecular Biology, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
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  • S.H.E. Lim

    Corresponding author
    1. Department of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia
    • Correspondence

      Swee Hua Erin Lim, School of Pharmacy, Department of Life Sciences, International Medical University, No. 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.

      E-mail: erin_lim@imu.edu.my

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Abstract

Aim

The aim of this study was to investigate the mode of action of the lavender essential oil (LV) on antimicrobial activity against multi-drug-resistant Escherichia coli J53 R1 when used singly and in combination with piperacillin.

Method and Results

In the time-kill analysis, a complete killing of bacteria was observed based on colony counts within 4 h when LV was combined with piperacillin during exposure at determined FIC concentrations. Analysis of the membrane permeabilizing effects of LV on treated cultures through their stability against sodium dodecyl sulphate revealed that the LV played a role in disrupting the bacterial cell membrane. The finding is further supported by scanning electron microscopy analysis and zeta potential measurement. In addition, reduction in light production expression of E. coli [pSB1075] by the LV showed the presence of potential quorum sensing (QS) inhibitors.

Conclusions

These results indicated that the LV has the potential to reverse bacterial resistance to piperacillin in E. coli J53 R1. It may operate via two mechanisms: alteration of outer membrane permeability and inhibition of bacterial QS.

Significance and Impact of the Study

These findings offer a novel approach to develop a new option of phytopharmaceuticals against multi-drug-resistant E. coli.

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