Antimicrobial activity of Metschnikowia pulcherrima on wine yeasts
Article first published online: 13 FEB 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 5, pages 1209–1217, May 2014
How to Cite
Oro, L., Ciani, M. and Comitini, F. (2014), Antimicrobial activity of Metschnikowia pulcherrima on wine yeasts. Journal of Applied Microbiology, 116: 1209–1217. doi: 10.1111/jam.12446
- Issue published online: 11 APR 2014
- Article first published online: 13 FEB 2014
- Accepted manuscript online: 21 JAN 2014 04:15AM EST
- Manuscript Accepted: 9 JAN 2014
- Manuscript Revised: 4 DEC 2013
- Manuscript Received: 8 OCT 2013
- antimicrobial compounds;
- Metschnikowia pulcherrima;
- mixed fermentation;
- yeast interactions
In the present study, it was investigated the antagonistic behaviour of Metschnikowia pulcherrima, as biocontrol agent, against the main wine yeast species involved in the winemaking process.
Methods and Results
Seven strains of M. pulcherrima were evaluated for the antimicrobial activity against 114 yeast strains belonging to Pichia, Candida, Hanseniaspora, Kluyveromyces, Saccharomycodes, Torulaspora, Brettanomyces and Saccharomyces genera. Results showed both different inter-generic and intra-generic responses to the antimicrobial action of M. pulcherrima strains. Interestingly, the antimicrobial activity of M. pulcherrima did not have any influence on the growth of Saccharomyces cerevisiae. Instead, M. pulcherrima displayed a broad and effective antimicrobial action on undesired wild spoilage yeasts, such as Brettanomyces/Dekkera, Hanseniaspora and Pichia genera. Fermentation trials carried out in synthetic grape must confirmed the antimicrobial activity of M. pulcherrima, determining the early death of the non-Saccharomyces co-inoculated cultures.
The antimicrobial activity of M. pulcherrima does not seem due to proteinaceous compounds such as killer phenomenon, but to the pulcherriminic acid (the precursor of pulcherrimin pigment) that depletes iron present in the medium, making it not available to the other yeasts.
Significance and Impact of the Study
These data agree with and further support the potential use of selected M. pulcherrima strains in controlled multistarter fermentations with S. cerevisiae starter cultures.