Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens
Article first published online: 8 FEB 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 5, pages 1084–1093, May 2014
How to Cite
Braem, G., Stijlemans, B., Van Haken, W., De Vliegher, S., De Vuyst, L. and Leroy, F. (2014), Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens. Journal of Applied Microbiology, 116: 1084–1093. doi: 10.1111/jam.12447
- Issue published online: 11 APR 2014
- Article first published online: 8 FEB 2014
- Accepted manuscript online: 21 JAN 2014 04:15AM EST
- Manuscript Accepted: 8 JAN 2014
- Manuscript Revised: 28 DEC 2013
- Manuscript Received: 15 NOV 2013
- Research Council of the Vrije Universiteit Brussel
- Hercules Foundation
- Agency for Innovation by Science and Technology in Flanders
- IWT-Vlaanderen. Grant Number: 60714
- Staphylococcus ;
- udder health
To explore antibacterial activities of coagulase-negative staphylococci (CoNS) from teat apices of dairy cows towards mastitis-causing pathogens.
Methods and Results
Of 254 CoNS, 38 displayed bacteriocin-like activity after a first screening. Seven of these strains displayed activity against at least one mastitis-related pathogen (Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus). Staphylococcus chromogenes L217 displayed the strongest inhibitory effect, being active against all tested mastitis-related pathogens and most tested CoNS. Based on cation exchange and reversed-phase chromatography, in addition to N-terminal Edman degradation and PCR, the antibacterial peptide was identified as a nukacin-type bacteriocin and named nukacin L217. Although staphylococcal bacteriocins are generally found in the cell-free supernatants of liquid cultures, Staph. chromogenes L217 only led to detectable activity when grown on agar medium.
Bacteriocin-like activities are not uncommon among CoNS from teat apices and may inhibit mastitis-causing pathogens, as found for nukacin L217 production by Staph. chromogenes L217.
Significance and Impact of the Study
Nukacin L217 is the first identified bacteriocin of the species Staph. chromogenes and displays unusual production kinetics, that is, requiring surface growth of its producer. The fact that nukacins are produced by different CoNS species suggests a role in the teat skin ecosystem.