Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China
Article first published online: 24 MAR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
How to Cite
Li, D., Gu, A. Z. and He, M. (2014), Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China. Journal of Applied Microbiology. doi: 10.1111/jam.12456
- Article first published online: 24 MAR 2014
- Accepted manuscript online: 28 JAN 2014 08:47AM EST
- Manuscript Accepted: 20 JAN 2014
- Manuscript Revised: 18 JAN 2014
- Manuscript Received: 11 OCT 2013
- National Natural Science Foundation of China. Grant Number: 51178242
- National High Technology Research and Development Program (“863” Program) of China. Grant Numbers: 2014AA06A506, 2014AA06A506
- genetic diversity;
- mcyA ;
- Microcystis ;
- quantitative PCR
The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes.
Methods and Results
Abundances of total and microcystin-producing Microcystis varied across sampling periods and locations, which were assessed using qPCR with primers specific to Microcystis 16S rDNA and mcyA genes. The 16S rDNA from two lakes were relatively diverse. However, mcyA genes were rather conservative and were >97% identical to reference sequences. The highly positive correlations between mcyA and microcystin presence (r = 0·671 in Tai; r = 0·799 in Yang-cheng) suggested that mcyA can be used as a good biomarker for microcystin productions.
The results demonstrated that Microcystis were genetically diverse between these conjunctive lakes; however, mcyA genes were relatively conservative in two lakes. Quantifying mcyA by qPCR was an efficient tool for monitoring toxic Microcystis.
Significance and Impact of the Study
This study has improved our understanding of observable differences within and between each lake on spatial and temporal scales. And the discovery of new mcyA sequences in natural water enriched the understanding of phylogenetic diversity of Microcystis and toxin-production-related mcy gene.