Bacillus anthracis lethal toxin induces cell-type-specific cytotoxicity in human lung cell lines
Article first published online: 27 FEB 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 5, pages 1334–1343, May 2014
How to Cite
Kim, N.Y., Kang, C.I., Hur, G.H., Yang, J.M. and Shin, S. (2014), Bacillus anthracis lethal toxin induces cell-type-specific cytotoxicity in human lung cell lines. Journal of Applied Microbiology, 116: 1334–1343. doi: 10.1111/jam.12457
- Issue published online: 11 APR 2014
- Article first published online: 27 FEB 2014
- Accepted manuscript online: 29 JAN 2014 02:55AM EST
- Manuscript Accepted: 23 JAN 2014
- Manuscript Revised: 13 JAN 2014
- Manuscript Received: 15 SEP 2013
- inhalational anthrax;
- lethal toxin;
- lung cells
Inhalational anthrax is caused by the entry of Bacillus anthracis spores into the lung. Inhaled spores are phagocytosed by alveolar macrophages. Bacilli then escape from the macrophage and spread to other cells, initiating a systemic anthrax infection. Based on the pathological studies of primate and human inhalational anthrax cases, it appears that lung tissue injury is a lethal consequence of the disease. Although the cytotoxicity of anthrax lethal toxin to macrophages is well known, it is not clear how anthrax toxin affects the various lung cell types.
Methods and Results
Using model cell lines representing different physiological compartments of the lung, we have investigated the cytotoxic effects of anthrax lethal toxin. The cell response was evaluated through MTT metabolism, neutral red uptake, initiation of apoptosis, and expression and binding activity of anthrax toxin receptors. We found that a human small airway epithelial cell line, HSAEC, was susceptible to anthrax lethal toxin. The other cell lines, A549, MRC-5, H358 and SKLU-1, displayed resistance to anthrax lethal toxin-mediated toxicity, although the expression of anthrax toxin receptors was detected in all the cell lines tested.
Our results indicate that cell-type-specific toxicity may be induced by anthrax lethal toxin in human lung tissues and does not correlate with anthrax toxin receptor expression levels.
Significance and Impact of the Study
This work suggests that cell-type-specific cytotoxicity of anthrax toxin in lung cells may cause subsequent lung disease progression. It may explain the initial pathogenic step of inhalational anthrax.