Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms
Article first published online: 21 FEB 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 5, pages 1164–1171, May 2014
How to Cite
Asahi, Y., Noiri, Y., Miura, J., Maezono, H., Yamaguchi, M., Yamamoto, R., Azakami, H., Hayashi, M. and Ebisu, S. (2014), Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms. Journal of Applied Microbiology, 116: 1164–1171. doi: 10.1111/jam.12458
- Issue published online: 11 APR 2014
- Article first published online: 21 FEB 2014
- Accepted manuscript online: 29 JAN 2014 02:55AM EST
- Manuscript Accepted: 22 JAN 2014
- Manuscript Revised: 15 JAN 2014
- Manuscript Received: 11 NOV 2013
- Japan Science and Technology Agency. Grant Number: #24792020
- Japan Society for the Promotion of Science
- Nanotechnology Platform of the Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Research Center for Ultra-High Voltage Electron Microscopy
- Osaka University
- epigallocatechin gallate;
- Porphyromonas gingivalis ;
The aim of this study was to investigate the effects of tea catechin epigallocatechin gallate (EGCg) on established biofilms and biofilm formation by Porphyromonas gingivalis, a major pathogen of periodontal disease.
Methods and Results
Biofilm cell survival was measured using adenosine triphosphate (ATP) bioluminescence. In the presence of EGCg, the ATP level in cells of established biofilms was significantly decreased compared to the controls (P < 0·0001). Transmission electron microscopy revealed that EGCg damaged the cell membrane and cell wall of P. gingivalis. Confocal laser-scanning microscopy revealed that the proportion of dead cells was higher in biofilms treated with EGCg. Moreover, the effects of subminimal inhibitory concentrations (MICs) of EGCg on P. gingivalis biofilm formation were dose-dependent (P < 0·0001).
Our results suggest that EGCg destroys established P. gingivalis biofilms and inhibits biofilm formation.
Significance and Impact of the Study
Development of chemical control agents against oral biofilms is necessary, because oral biofilms can be only removed using mechanical debridement. This article indicates that EGCg may represent a novel antibiofilm agent that prevents infections involving bacterial biofilms such as periodontitis.