Virulence factors and infection ability of Pseudomonas aeruginosa isolates from a hydropathic facility and respiratory infections
Article first published online: 25 FEB 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 5, pages 1359–1368, May 2014
How to Cite
Pereira, S.G., Rosa, A.C., Ferreira, A.S., Moreira, L.M., Proença, D.N., Morais, P.V. and Cardoso, O. (2014), Virulence factors and infection ability of Pseudomonas aeruginosa isolates from a hydropathic facility and respiratory infections. Journal of Applied Microbiology, 116: 1359–1368. doi: 10.1111/jam.12463
- Issue published online: 11 APR 2014
- Article first published online: 25 FEB 2014
- Accepted manuscript online: 3 FEB 2014 01:41AM EST
- Manuscript Accepted: 27 JAN 2014
- Manuscript Revised: 17 JAN 2014
- Manuscript Received: 7 AUG 2013
- Portuguese Foundation for Science and Technology. Grant Numbers: SFRH/BD/46668/2008, SFRH/76044/2009, PTDC/QUI-BIQ/118260/2010
- Center for Pharmaceutical Studies and FEDER
- infection control;
- Pseudomonas aeruginosa ;
- respiratory infections;
- waterborne infections
To compare the virulence pool and acute infection ability of Pseudomonas aeruginosa isolates from a hydropathic facility, used to treat respiratory conditions by inhalation of untreated natural mineral water, with clinical isolates from respiratory infections.
Methods and Results
Pseudomonas aeruginosa isolates from a hydropathic facility and from respiratory infections were typed by pulsed-field gel electrophoresis. Nonclonal representatives of each population were selected. 18 virulence-encoding genes were screened by polymerase chain reaction and statistically compared by multiple correspondence analysis. Homogeneous distribution of genes between populations but higher genetic association in aquatic isolates was observed, as well as distinct virulence pool according to location in the water system. Acute infection ability of selected isolates from each population, in Galleria mellonella model, showed lower LD50 of the majority of the hydropathic isolates and significant variations in LD50 of biofilm isolates from different equipments.
Hydrotherapy Ps. aeruginosa isolates present similar virulence to isolates from respiratory infections. Hydrotherapy users may be exposed to different microbiological risks when using different treatment equipments.
Significance and Impact of the Study
Twenty-one million people use hydropathic facilities in Europe, and the majority present risk factors to pneumonia. This study demonstrates the health risk associated with this practice. Revision of European regulations should be considered.