These authors contributed equally.
The endophyte Verticillium Vt305 protects cauliflower against Verticillium wilt
Article first published online: 13 MAR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 6, pages 1563–1571, June 2014
How to Cite
Tyvaert, L., França, S.C., Debode, J. and Höfte, M. (2014), The endophyte Verticillium Vt305 protects cauliflower against Verticillium wilt. Journal of Applied Microbiology, 116: 1563–1571. doi: 10.1111/jam.12481
- Issue published online: 19 MAY 2014
- Article first published online: 13 MAR 2014
- Accepted manuscript online: 20 FEB 2014 12:24AM EST
- Manuscript Accepted: 12 FEB 2014
- Manuscript Revised: 10 FEB 2014
- Manuscript Received: 12 DEC 2013
- Innovation by Science and Technology (IWT)
- biological control;
- Verticillium isaacii ;
- Verticillium longisporum ;
- Verticillium tricorpus
To investigate the interaction between cauliflower and the isolate VerticilliumVt305, obtained from a field suppressive to Verticillium wilt of cauliflower, and to evaluate the ability of VerticilliumVt305 to control Verticillium wilt of cauliflower caused by V. longisporum.
Methods and Results
Single and combined inoculations of VerticilliumVt305 and V. longisporum were performed on cauliflower seedlings. Symptom development was evaluated, and fungal colonization was measured in the roots, hypocotyl and stem with real-time PCR. No symptoms were observed after single inoculation of VerticilliumVt305, although it colonized the plant tissues. Pre-inoculation of VerticilliumVt305 reduced symptom development and colonization of plant tissues by V. longisporum.
VerticilliumVt305 is an endophyte on cauliflower plants and showed effective biological control of V. longisporum in controlled conditions.
Significance and Impact of the Study
This work can contribute to the development of a sustainable control measure of V. longisporum in Brassicaceae hosts, which is currently not available. Additionally, this study provides evidence for the different roles of Verticillium species present in the agro-ecosystem.