Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation
Article first published online: 24 MAR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 6, pages 1531–1542, June 2014
How to Cite
Sapaar, B., Nur, A., Hirota, K., Yumoto, H., Murakami, K., Amoh, T., Matsuo, T., Ichikawa, T. and Miyake, Y. (2014), Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation. Journal of Applied Microbiology, 116: 1531–1542. doi: 10.1111/jam.12483
- Issue published online: 19 MAY 2014
- Article first published online: 24 MAR 2014
- Accepted manuscript online: 20 FEB 2014 12:25AM EST
- Manuscript Accepted: 12 FEB 2014
- Manuscript Revised: 12 DEC 2013
- Manuscript Received: 20 AUG 2013
- Japan Society. Grant Numbers: 24592833, 24592872
- biofilm development;
- Candida albicans;
- extracellular DNA;
- genomic DNA;
- hyphal form
The aim of this study was to investigate the effects of genomic DNA purified from Candida albicans and pneumonia-related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, on in vitro biofilm formation and morphological change of 3 Candida species (C. albicans, C. glabrata, and C. tropicalis).
Methods and Results
Biofilm formation was evaluated by the crystal violet assay and colony-forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (<1·0 μg ml−1) significantly increased biofilm mass of all three Candida species. In contrast, the addition of DNA at a high concentration (10 μg ml−1) decreased the biofilm mass. Interestingly, the formation of hyphae in a dense network of yeast cells was observed in C. albicans biofilms exposed to a low concentration of DNA (<1·0 μg ml−1).
These findings demonstrated that extracellular DNA (eDNA) plays a crucial role in Candida biofilm formation and suggested that eDNA may induce the morphological transition from yeast to hyphal growth form during C. albicans biofilm development.
Significance and Impact of the Study
A novel therapy targeting eDNA may be applicable for Candida infection to decrease biofilm formation and hyphal formation.