Enhanced bactericidal action of acidified sodium chlorite caused by the saturation of reactants
Article first published online: 17 MAR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 6, pages 1447–1457, June 2014
How to Cite
Kim, N.H., Park, T.H. and Rhee, M.S. (2014), Enhanced bactericidal action of acidified sodium chlorite caused by the saturation of reactants. Journal of Applied Microbiology, 116: 1447–1457. doi: 10.1111/jam.12484
- Issue published online: 19 MAY 2014
- Article first published online: 17 MAR 2014
- Accepted manuscript online: 23 FEB 2014 05:13AM EST
- Manuscript Accepted: 18 FEB 2014
- Manuscript Revised: 4 FEB 2014
- Manuscript Received: 4 DEC 2013
- Korea University grant
- acidified sodium chlorite;
- bactericidal action;
- foodborne pathogen;
- initial concentration of reactants;
- preparation procedure
Factors affecting the antibacterial action of acidified sodium chlorite (ASC), a widely used disinfectant, have not been determined. This study investigated the significant factors suggesting efficient production method to maximize bactericidal action of ASC.
Methods and Results
The effects of (i) preparation procedures (total three methods); (ii) initial concentrations of reactants: sodium chlorite (SC) and citric acid (CTA) (up to maximum solubility of each reactant) and (iii) final pH values (3·0 and 2·5) to the bactericidal action of ASC were investigated with a fixed final concentration of SC (10 ppm) using various foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium and Staphylococcus aureus). The antimicrobial compounds produced and the bactericidal effects depended on the preparation procedure and the initial concentrations of the reactants. The ASC prepared by premixing highly concentrated reactants (in particular > 40%) followed by dilution (dilution after reaction, DAR) was more effective in inactivating foodborne pathogens, and it produced higher antimicrobial compound (Cl2 and ClO2) yields than the other procedures. A 5-min treatment with ASC, produced using the other procedures, resulted in a reduction of < 3·5 log CFU ml−1 (Gram positive = 0·18–0·78; Gram negative = 0·03–3·49 log CFU ml−1), whereas ASC produced with the DAR procedure using the saturated reactants completely inactivated all of the test pathogens within 5 min without recovery (initial concentration = 6·94–7·08 log CFU ml−1).
The ASC production with the DAR procedure using the saturated reactants maximizes both the antimicrobial compound yields and bactericidal effects of the ASC solutions.
Significance and Impact of the Study
This study will contribute to increase the efficiency of ASC treatments for disinfections reducing the effective SC concentrations for industrial use.