A novel echinocandin MIG0310 with anticandida activity from newly isolated Fusarium sp. strain MS-R1
Article first published online: 27 MAR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 116, Issue 6, pages 1458–1464, June 2014
How to Cite
Masaphy, S. (2014), A novel echinocandin MIG0310 with anticandida activity from newly isolated Fusarium sp. strain MS-R1. Journal of Applied Microbiology, 116: 1458–1464. doi: 10.1111/jam.12493
- Issue published online: 19 MAY 2014
- Article first published online: 27 MAR 2014
- Accepted manuscript online: 10 MAR 2014 03:07AM EST
- Manuscript Accepted: 5 MAR 2014
- Manuscript Revised: 27 FEB 2014
- Manuscript Received: 22 DEC 2013
- The Office of the Chief Scientist
- Israeli Ministry of Industry
- Trade and Labor. Grant Number: 832
- Candida albicans ;
- Fusarium ;
- strain MS-R1
To isolate and identify an anticandida compound from newly isolated Fusarium strain MS-R1 and characterization of its activity against standard and clinical strains of Candida.
Methods and Results
The fungal strain, Fusarium strain MS-R1, was isolated from soil. Molecular identification according to the internal transcribed spacer region of the rRNA gene sequence showed the strain to be strongly related to Fusarium brachygibbosum complex. Successive extractions of the active compound showed activity against Candida albicans, including clinical strains. Inhibition of a clinical strain of Candida tropicalis, but not Candida krusei and Candida glabrata, was also shown as tested by the broth microdilution assay. The compound was purified by liquid chromatography coupled with thin-layer chromatography and bioautography and characterized by nuclear magnetic resonance spectroscopy. It was confirmed to have the molecular formula C48H66O18 and was identified as an echinocandin with a novel structure.
A novel echinocandin-type antifungal metabolite, MIG0310, was isolated and characterized. This is a second echinocandin reported from a Fusarium species, indicating this genus to have wider range of echinocandin compounds.
Significance and Impact of the Study
The new compound may lead to new anticandidal drugs, broadening the treatment opportunities for Candida species, including those that are resistant to the current antifungal drugs.