Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model

Authors

  • J. Zhu,

    1. Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    2. Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China
    Search for more papers by this author
  • C. Zhu,

    1. Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    2. Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China
    Search for more papers by this author
  • S. Ge,

    1. Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    2. Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China
    Search for more papers by this author
  • M. Zhang,

    1. School of Food and Chemical Engineering, Beijing Technology and Business University, China
    Search for more papers by this author
  • L. Jiang,

    1. Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    2. Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China
    Search for more papers by this author
  • J. Cui,

    1. Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    2. Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China
    Search for more papers by this author
  • F. Ren

    Corresponding author
    1. Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    2. Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China
    • Correspondence

      Fazheng Ren, China Agricultural University, P.O. Box 287, No. 17 Qinghua East Road, Beijing 100083, China.

      E-mail: renfazheng@263.net

    Search for more papers by this author

Abstract

Aims

The objective of this study was to investigate the impact of Lactobacillus salivarius Ren (LS) on modulating colonic micro flora structure and influencing host colonic health in a rat model with colorectal precancerous lesions.

Methods and Results

Male F344 rats were injected with 1, 2-dimethylhydrazine (DMH) and treated with LS of two doses (5 × 108 and 1 × 1010 CFU kg−1 body weight) for 15 weeks. The colonic microflora profiles, luminal metabolites, epithelial proliferation and precancerous lesions [aberrant crypt foci (ACF)] were determined. A distinct segregation of colonic microflora structures was observed in LS-treated group. The abundance of one Prevotella-related strain was increased, and the abundance of one Bacillus-related strain was decreased by LS treatment. These changes were accompanied by increased short-chain fatty acid levels and decreased azoreductase activity. LS treatment also reduced the number of ACF by c. 40% and suppressed epithelial proliferation.

Conclusions

Lactobacillus salivarius Ren improved the colonic microflora structures and the luminal metabolisms in addition preventing the early colorectal carcinogenesis in DMH-induced rat model.

Significance and Impact of the Study

Colonic microflora is an important factor in colorectal carcinogenesis. Modulating the structural shifts of microflora may provide a novel option for preventing colorectal carcinogenesis. This study suggested a potential probiotic-based approach to modulate the intestinal microflora in the prevention of colorectal carcinogenesis.

Ancillary