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Investigation into in vitro and in vivo models using intestinal epithelial IPEC-J2 cells and Caenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic Escherichia coli

Authors

  • M. Zhou,

    1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China
    2. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada
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  • J. Zhu,

    1. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada
    2. Laboratory of Molecular Microbial Ecology and Ecogenomics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
    Current affiliation:
    1. WILEY-BLACKWELL, John Wiley & Sons (Asia) Pte Ltd, Shanghai, China
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  • H. Yu,

    1. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada
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  • X. Yin,

    1. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada
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  • P.M. Sabour,

    1. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada
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  • L. Zhao,

    1. Laboratory of Molecular Microbial Ecology and Ecogenomics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
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  • W. Chen,

    Corresponding author
    1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China
    • Correspondence

      Joshua Gong, 93 Stone Road, Guelph, ON, N1G 5C9 Canada.

      E-mail: joshua.gong@agr.gc.ca and

      Wei Chen, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

      E-mail: chenwei66@jiangnan.edu.cn

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  • J. Gong

    Corresponding author
    1. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada
    • Correspondence

      Joshua Gong, 93 Stone Road, Guelph, ON, N1G 5C9 Canada.

      E-mail: joshua.gong@agr.gc.ca and

      Wei Chen, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

      E-mail: chenwei66@jiangnan.edu.cn

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Abstract

Aims

To identify a fast, economic and reliable method for preselecting lactic acid-producing bacterial (LAB) isolates to control enterotoxigenic Escherichia coli (ETEC).

Methods and Results

Two assays with porcine intestinal epithelial IPEC-J2 cells or Caenorhabditis elegans for selecting effective probiotic candidates were compared. Both assays were based on measuring death of cells or worms caused by ETEC strain JG280. Six of 13 LAB isolates showed ≥50% protection in each assay, among which only four isolates (≥50% protection) were consistently selected by both assays. Isolate CL9 (Lactobacillus reuteri) was further studied. It reduced gene expression of estA, estB and elt in JG280 in both assays. Furthermore, the isolate protected IPEC-J2 and C. elegans from cell and worm death caused by STa, STb or LT enterotoxin expressed in E. coli DH5α. CL9 also promoted host defensive responses by decreasing IL-8 and increasing IL-10 production in IPEC-J2 cells and expression of antimicrobial peptide genes clec-60, clec-85 in C. elegans.

Conclusions

Caenorhabditis elegans is useful for preselecting probiotic candidates to control ETEC after initial screening with IPEC-J2 cells.

Significance and Impact of the Study

A combination of IPEC-J2 cell and C. elegans assays can improve the effectiveness in preselecting probiotic candidates.

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