Effects of salinity on antibiotic production in sponge-derived Salinispora actinobacteria
Article first published online: 19 APR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 1, pages 109–125, July 2014
How to Cite
Ng, Y.K., Hodson, M.P., Hewavitharana, A.K., Bose, U., Shaw, P.N. and Fuerst, J.A. (2014), Effects of salinity on antibiotic production in sponge-derived Salinispora actinobacteria. Journal of Applied Microbiology, 117: 109–125. doi: 10.1111/jam.12507
- Issue published online: 16 JUN 2014
- Article first published online: 19 APR 2014
- Accepted manuscript online: 29 MAR 2014 01:25AM EST
- Manuscript Accepted: 14 MAR 2014
- Manuscript Revised: 2 MAR 2014
- Manuscript Received: 3 DEC 2013
- Australian Research Council (ARC)
- University of Queensland Research Scholarship (UQRS)
- University of Queensland International Research Tuition Award (UQIRTA)
- Australian Government (NCRIS)
- Queensland State Government
- University of Queensland
- stress response
To investigate the effects of growth conditions related to marine habitat on antibiotic production in sponge-derived Salinispora actinobacteria.
Methods and Results
Media with varying salt concentration were used to investigate the effects of salinity in relation to Salinispora growth and rifamycin production. The chemotypic profiles of the model strain Salinispora arenicola M413 was then assessed using metabolomic fingerprints from high-pressure liquid chromatography with diode array detection (HPLC-DAD) and multivariate data analysis, before extending this approach to two other strains of S. arenicola. Fingerprint data were generated from extracts of S. arenicola broth cultures grown in media of varying salt (NaCl) concentrations. These fingerprints were then compared using multivariate analysis methods such as principal components analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). From the analysis, a low-sodium growth condition (1% NaCl) was found to delay the onset of growth of the model S. arenicola M413 strain when compared to growth in media with either 3% artificial sea salt or 3% NaCl. However, low-sodium growth conditions also increased cell mass yield and contributed to at least a significant twofold increase in rifamycin yield when compared to growth in 3% artificial sea salt and 3% NaCl.
The integration of HPLC-DAD and multivariate analysis proved to be an effective method of assessing chemotypic variations in Salinispora grown in different salt conditions, with clear differences between strain-related chemotypes apparent due to varying salt concentrations.
Significance and Impact of the Study
The observed variation in S. arenicola chemotypic profiles further suggests diversity in secondary metabolites in this actinomycete in response to changes in the salinity of its environment.