Enhancement of Lactobacillus reuteri KUB-AC5 survival in broiler gastrointestinal tract by microencapsulation with alginate–chitosan semi-interpenetrating polymer networks
Article first published online: 28 APR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 1, pages 227–238, July 2014
How to Cite
Rodklongtan, A., La-ongkham, O., Nitisinprasert, S. and Chitprasert, P. (2014), Enhancement of Lactobacillus reuteri KUB-AC5 survival in broiler gastrointestinal tract by microencapsulation with alginate–chitosan semi-interpenetrating polymer networks. Journal of Applied Microbiology, 117: 227–238. doi: 10.1111/jam.12517
- Issue published online: 16 JUN 2014
- Article first published online: 28 APR 2014
- Accepted manuscript online: 8 APR 2014 01:00PM EST
- Manuscript Accepted: 1 APR 2014
- Manuscript Revised: 31 MAR 2014
- Manuscript Received: 17 FEB 2014
- Kasetsart University Research and Development Institute (KURDI)
- Betagro Science Center Co., Ltd., Thailand
- gastrointestinal tract;
- Lactobacillus reuteri KUB-AC5;
To evaluate an alginate–chitosan microcapsule for an intestinal probiotic delivery system for broilers.
Methods and Results
Lactobacillus reuteri KUB-AC5 was successfully microencapsulated with alginate and chitosan mixtures using an emulsion cross-linking method with high microencapsulation efficiency. Scanning electron microscopy revealed a large number of the bacteria entrapped in the semi-interpenetrating network. The microcapsule effectively protected the cells against strong acids. The in vitro study showed that the 8 log CFU g−1 was released at the jejunum and ileum. For the in vivo study, the number of probiotics was detected by a polymerase chain reaction-based random amplified polymorphic DNA technique. From provision of 10 log CFU, cell numbers of 5–8 log CFU were observed in the intestine.
The alginate–chitosan microcapsule can serve as a potential intestine-targeted probiotic delivery system.
Significance and Impacts of the Study
To the best of our knowledge, this is the first comparison study of the in vitro and in vivo gastrointestinal profiles of microencapsulated probiotics used as feed additives for broilers. This study reveals the similarities and differences of the in vitro and in vivo probiotic profiles and provides convincing evidence of the potential use of the alginate–chitosan microcapsule as a probiotic delivery system.