Assessment of stress tolerance acquisition in the heat-tolerant derivative strains of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG
Article first published online: 28 APR 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 1, pages 239–248, July 2014
How to Cite
Aakko, J., Sánchez, B., Gueimonde, M. and Salminen, S. (2014), Assessment of stress tolerance acquisition in the heat-tolerant derivative strains of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG. Journal of Applied Microbiology, 117: 239–248. doi: 10.1111/jam.12520
- Issue published online: 16 JUN 2014
- Article first published online: 28 APR 2014
- Accepted manuscript online: 11 APR 2014 12:15PM EST
- Manuscript Accepted: 3 APR 2014
- Manuscript Revised: 2 APR 2014
- Manuscript Received: 31 OCT 2013
- TEKES (the Finnish Funding Agency for Technology and Innovation). Grant Number: 398/31/2009
- Bifidobacterium animalis subsp. lactis BB-12;
- heat shock;
- Lactobacillus rhamnosus GG ;
The purpose of this study was to investigate the heat-shock response at molecular level in Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. lactis BB-12 and their heat-tolerant derivatives and to characterize the changes that make the derivatives more robust in terms of heat stress.
Methods and results
The study strains were exposed for 2 h to a heat-shock treatment, Bif. animalis subsp. lactis BB-12 and its derivative at 50°C and the Lact. rhamnosus GG and its derivative at 60°C. Protein synthesis before and after heat shock was examined using proteomics and RT-qPCR. The analysis revealed that the regulation of seven proteins in both strain pairs was modified as a response to heat or between the original and the derivative strain. The comparison of wild-type strains and the heat-tolerant derivatives suggests that the acquisition of heat tolerance in the Bif. animalis subsp. lactis BB-12 derivative is due to a slightly increased constitutive level of chaperones, while in Lact. rhamnosus GG derivative, the main reason seems to be a higher ability to induce the production of chaperones.
This study revealed possible markers of heat tolerance in B. lactis and Lact. rhamnosus strains.
Significance and impact of study
This study increases our knowledge on how Lactobacillus and Bifidobacterium strains may acquire heat tolerance. These findings may be useful for improving the heat tolerance of existing probiotic strains as well as screening new heat-tolerant strains.