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Two figures in PDF format, about specificity of E. coli O157:H7 biosensor and how to determine the time threshold of detection analysis are available in supplemental information.

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jam12522-sup-0001-FigS1-S2.docxWord document1773K

Figure S1 Specificity of Escherichia coli O157:H7 biosensor. (image)Ecp: anti-E. coli O157:H7 polyclonal antibodies; (image) Ecm: anti-E. coli O157:H7 monoclonal antibodies; (▲▲▲) NC: negative control antibodies. (a) Culture of Salmonella Enteritidis (455 ± 271 CFU ml−1). (b) Culture of E. coli O26:H32 (339 ± 135 CFU ml−1). (c) Culture of E. coli O121.H19 (453 ± 91 CFU ml−1). (d) Culture of E. coli O103 (128 ± 55 CFU ml−1). E. coli O26:H32, E. coli O121.H19 and E. coli O103 were provided by P. Fach team at ANSES and were cultivated in same conditions than E. coli O157:H7.

Figure S2 Determination of the Time threshold (Tt). The Tt correspond to the time necessary to increase the signal of an average of three curves corresponding to the reflectivity of three Ecp spots on the array, by 1% from the ΔR of the trend curve. A ΔR > 1% is significant for bacterial detection, it is largely superior to the SPR detection threshold (Bouguelia et al. 2013; Daniel et al. 2013).

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