Rhizospheric fungal community structure of a Bt brinjal and a near isogenic variety
Article first published online: 11 JUN 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 3, pages 750–765, September 2014
How to Cite
Singh, A.K., Singh, M. and Dubey, S.K. (2014), Rhizospheric fungal community structure of a Bt brinjal and a near isogenic variety. Journal of Applied Microbiology, 117: 750–765. doi: 10.1111/jam.12549
- Issue published online: 18 AUG 2014
- Article first published online: 11 JUN 2014
- Accepted manuscript online: 22 MAY 2014 03:19AM EST
- Manuscript Revised: 17 MAY 2014
- Manuscript Accepted: 17 MAY 2014
- Manuscript Received: 2 APR 2014
- Indian Institute of Vegetable Research, India
- Bt brinjal;
- Cry1Ac gene;
- fungal communities;
The objective of this study was to investigate the influence of Cry1Ac gene expressing brinjal (VRBT-8) on the rhizospheric fungal community structure.
Methods and Results
qPCR indicated variations in the fungal ITS rRNA copy numbers of non-Bt (1·43–4·43) × 109 g−1 dws and Bt (1·43–3·32) × 109 g−1 dws plots. Phylogenetic analysis of ITS rRNA clones indicated fungal-related group majority of being Ascomycota compared to that of Basidiomycota and Zygomycota in non-Bt- and Bt-planted soils. Sordariomycetes was the dominant class detected in all the stages.
Despite the variations in the population size and the distribution pattern observed across the non-Bt and Bt brinjal, plant-growth-dependent variability was more prominent compared with genetic modification. Therefore, this study concludes that genetic modification of brinjal crop has minor effect on the fungal community.
Significance and Impact of the Study
Brinjal, the important solanaceous crop, is also prone to attack by many insect pests, especially by Leucinoides orbonalis, resulting in significant losses in the crop yield. However, the reports on the effect of transgenic crops and the associated microbial community are inconsistent. The present communication takes into account for the first time the possible interactions between Bt brinjal and the associated fungal community; the latter playing a significant role in maintaining soil fertility. As this study is limited to the structural diversity of fungal community, additional information regarding the functional diversity of the group seems imperative before recommending the commercialization of GM crops.